苏云金杆菌亚致死浓度对绿豆象种群繁殖的影响  

Bacillus thuringiensis Sublethal Concentration: Effects on Population Reproduction of Callosobruchus chinensis(Linnaeus)

作　　者：杨佳乐[1] 马新[1] 曹敏 余慧萍 杨倩[1] 张李香[1] Yang Jiale;Ma Xin;Cao Min;Yu Huiping;Yang Qian;Zhang Lixiang(College of Modern Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080)

机构地区：[1]黑龙江大学现代农业与生态环境学院,哈尔滨150080

出　　处：《中国农学通报》2020年第24期132-135,共4页Chinese Agricultural Science Bulletin

基　　金：黑龙江大学学科青年学术骨干百人支持计划项目;黑龙江省高校基本科研业务费黑龙江大学专项资金项目(HDJCCX-201617)。

摘　　要：为明确苏云金芽孢杆菌对绿豆象[Callosobruchus chinensis(Linnaeus)]种群繁殖力的影响,采用浸渍绿豆法对绿豆象成虫开展室内生测。结果显示:苏云金杆菌的剂量分别为80.0 IU/mg、53.3 IU/mg、32.0 IU/mg、26.7 IU/mg、20.0 IU/mg时,绿豆象成虫的致死率依次表现为95.56%、70.00%、36.67%、21.11%、7.78%,其毒力回归方程为y=0.38x+2.91。苏云金杆菌亚致死剂量LC10、LC30、LC50可明显缩短绿豆象成虫的寿命、抑制雌成虫的产卵能力。亚致死剂量处理组绿豆象种群的净增值率、内禀增长率和周限增长率的值均低于对照组,亚致死剂量处理组种群的加倍时间显著延长。由此可见,苏云金杆菌对绿豆象有较好的杀虫效果,亚致死剂量对绿豆象种群的发育有明显的抑制作用。In order to determine the effect of Bacillus thuringiensis on the population reproduction of Callosobruchus chinensis, a laboratory bioassay was conducted on the adults of Callosobruchus chinensis bymacerating method. The results showed that the fatality rates of adults were 95.56%, 70.00%, 36.67%,21.11%, 7.78%, respectively, when the doses of Bacillus thuringiensis were 80.0 IU/mg, 53.3 IU/mg, 32.0 IU/mg,26.7 IU/mg, 20.0 IU/mg, respectively. Its virulence regression equation was y=0.38 x+2.91. LC10, LC30 and LC50 significantly shortened the lifespan of the adults of Callosobruchus chinensis and inhibited the oviposition offemale adults. After being treated by LC10, LC30 or LC50, the net reproduction rate, intrinsic growth rate and finiterate of increase were lower than that of the control, while the population doubling time was prolonged obviously.It indicates that Bacillus thuringiensis has a good insecticidal effect, and the sublethal dose has a significantinhibiting effect on the population development of Callosobruchus chinensis.

关 键 词：绿豆象 苏云金芽孢杆菌 亚致死浓度 繁殖力 

分 类 号：S4[农业科学—植物保护]