重组酶聚合酶等温扩增技术在动物源性成分鉴定中的应用  

Recombinase-Polymerase Mediated Isothermal Amplification Technology: Application in Identification of Animal-Derived Ingredients

作　　者：王伊琴 杨帆 郭文丽 陈少博 包勇敢 荆文魁 张志峰 常鸿 康晓平 Wang Yiqin;Yang Fan;Guo Wenli;Chen Shaobo;Bao Yonggan;Jing Wenkui;Zhang Zhifeng;Chang Hong;Kang Xiaoping(Erenhot Customs Technology Center,Erenhot Inner Mongolia 011100;Epidemic Prevention and Control Center of Erenhot,Erenhot Inner Mongolia 011100;Hohhot Customs,Hohhot 010010;State Key Laboratory of Pathogen and Biosecurity,Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071)

机构地区：[1]二连海关技术中心,内蒙古二连浩特011100 [2]二连浩特市疾病预防控制中心,内蒙古二连浩特011100 [3]呼和浩特海关,呼和浩特010010 [4]军事科学院军事医学研究院微生物流行病研究所,北京100071

出　　处：《中国农学通报》2020年第30期151-157,共7页Chinese Agricultural Science Bulletin

基　　金：海关总署科研计划项目“几种重要野生动物DNA快速鉴定关键技术的研究”(2019HK042);传染病重大专项“病毒感染高通量快速检测与应急筛检技术研究”(2018zx10711001-003-001)。

摘　　要：在打击肉制品掺假走私、维护市场秩序、保护野生动物资源等方面,对动物源性成分鉴定技术的特异性、灵敏性、重复性提出了很高的要求。本文阐述了动物源性成分的检测鉴定技术,有多样性和局限性的特点。归纳了重组酶聚合酶恒等温扩增技术原理与应用,解决了PCR技术在仪器上的局限性,优化了早期恒温扩增技术的缺点。指出重组酶聚合酶恒等温扩增技术为动物源性成分的快速准确鉴定提供了坚实的技术基础,并在动物疫病诊断方面也有很好的应用前景。The identification technology of animal-derived ingredients requires high specificity, sensitivity and repeatability of detection methods in combating adulteration and smuggling of meat products, maintaining market orders and protecting wildlife resources. In this study, the detection and identification technologies of animal-derived ingredients were described, which had the characteristics of diversity and limitation. The principle and application of recombinase-polymerase mediated isothermal amplification technology were summarized, the limitations of PCR technology on instruments were solved, and the early isothermal amplification technology was optimized. The study points out that the recombinase-polymerase mediated isothermal amplification technology could be a technical basis for the rapid and accurate identification of animal-derived ingredients, and has a good application prospect in the diagnosis of animal epidemics.

关 键 词：重组酶聚合酶扩增技术 等温扩增 动物源性成分 鉴定 

分 类 号：S851.67[农业科学—预防兽医学]