熊果酸预处理对急性心肌梗死大鼠心功能和心肌病理改变的影响  

Effects of Ursolic Acid Preconditioning on Cardiac Function and Myocardial Pathology in Rats with Acute Myocardial Infarction

作　　者：王金艳[1] 肖亚利[1] 陈丽东 宋红 赵敏[1] 王国龙[1] Wang Jinyan;Xiao Yali;Chen Lidong(Department of Cardiovascular Medicine,Zhuozhou Hospital,Hebei 072750,China)

机构地区：[1]涿州市医院心血管内科,072750

出　　处：《医学研究杂志》2020年第10期88-91,104,共5页Journal of Medical Research

基　　金：河北省医学科学研究课题计划项目(20191796)。

摘　　要：目的探索熊果酸(UA)预处理对大鼠急性心肌梗死(AMI)后心功能和心肌组织病理改变的影响。方法180只实验用大鼠按照数字表法随机分为假手术组(Sham组),AMI组,UA低、中、高剂量(10、20、40mg/kg)组和维拉帕米(Ver,2.5mg/kg)预处理组,每组30只;各组于造模前3天开始1次/天腹腔注射给药,Sham组和AMI组给予0.9%氯化钠溶液;通过结扎左冠状动脉前降支制备AMI大鼠模型。造模手术24h后,心脏多普勒超声检测心功能指标,NBT染色法检测心肌梗死体积,HE染色法行左心室心肌病理学检查并评分,TUNEL法观察细胞凋亡,透射电子显微镜观察心肌细胞超微结构变化。结果与AMI组比较,UA中、高剂量和Ver预处理组LVIDd、LVIDs降低且EF、SV升高,心肌梗死体积降低,心肌组织病理性形态结构改变和细胞凋亡状况明显改善,损伤评分和凋亡指数(AI)显著降低,细胞超微结构病变明显改善;与Ver预处理组比较,UA高剂量预处理组LVIDs降低且SV升高,心肌梗死体积、损伤评分和AI降低;差异均有统计学意义(P<0.05或P<0.01)。结论UA预处理对AMI大鼠心功能和左心室心肌病理学改变及细胞超微结构病变具有保护作用。Objective To study the effect of Ursolic acid(UA)preconditioning on cardiac function and myocardial pathology in rats with acute myocardial infarction(AMI).Methods Totally 180 experimental rats were randomly divided into Sham group,AMI group,UA low-,medium-and high-dose(10,20,40mg/kg)and Verapami(Ver)2.5 mg/kg pretreatment group(n=30).Three days d before operation,the drugs were given by intraperitoneal injection once a day,the rats in Sham group and AMI group were given saline solution.The AMI rat model was established by ligating the left anterior descending coronary artery.24h after surgery,the cardiac function index were detected by cardiac doppler ultrasound.The volume of myocardial infarction was detected by NBT staining.The histopathological changes was observed by HE staining and injury score was calculated.The cardiomyocytes apoptosis was observed by TUNEL.The ultrastructure of cardiomyocytes was observed by TEM.Results Compared with AMI group,the LVIDd,LVIDs of the rats in UA medium-,high-dose and Ver pretreatment group decreased and the EF,SV increased.The percentage of infarct volume decreased,the morphological and structural changes of myocardial histopathology and apoptosis were significantly improved,and the injury score and apoptosis index(AI)decreased significantly.The ultrastructural changes of the cells were obviously improved.Compared with Ver pretreatment group,the LVIDs of the rats in UA high-dose pretreatment group decreased and the SV increased.The infarct volume,injury score and AI decreased.All of the differences were significant(P<0.05 or P<0.01).Conclusion UA pretreatment has protective effects on cardiac function,morphological and ultrastructural changes of left ventricular myocardium in AMI rats.

关 键 词：熊果酸 急性心肌梗死 心功能 病理 超微结构 

分 类 号：R543.3[医药卫生—心血管疾病]