微流控毛细管电泳系统用于Duffy血型基因分型研究  

Genotyping of Duffy Blood Group by Microchip Capillary Electrophoresis

作　　者：吴凡[1] 梁爽 庄乃保[1] 梁延连[1] 苏宇清[1] 洪文旭[1] WU Fan;LIANG Shuang;ZHUANG Nai-Bao;LIANG Yan-Lian;SU Yu-Qing;HONG Wen-Xu(Shenzhen Blood Center,Shenzhen 518035,Guangdong Province,China)

机构地区：[1]深圳市血液中心,广东深圳518035

出　　处：《中国实验血液学杂志》2020年第5期1733-1739,共7页Journal of Experimental Hematology

基　　金：深圳市科技创新委基础研究项目(JCYJ20170412150719814,JCYJ20180302153439758);深圳市卫生计生系统科研项目(SZGW2018008);深圳市医疗卫生三名工程项目(SZSM201811092);深圳市医学重点学科(SZXK070);中国输血协会威高基金(CSBT-WG-2018-09);广东省医学科学技术研究基金项目(A2020511)。

摘　　要：目的:探索快捷筛选分析Duffy血型基因型的方法,建立稀有血型献血者信息档案。方法:2014年12月-2018年9月本中心采用微流控毛细管电泳系统和PCR-SSP法检测3936名无血缘关系O型献血者标本Duffy基因型,对Fy^a阴性标本进行血清学确认及其他血型系统表型分型,Fy(a-b-)标本做基因测序。结果:Fy^a阴性标本血清学表型与微流控毛细管电泳系统基因分型结果一致。3936例样本中Duffy表型结果为Fy(a+b-)3564例(90.54%),Fy(a+b+)353例(8.97%),Fy(a-b+)18例(0.46%),Fy(a-b-)1例(0.03%)。基因频率:FY*A为95.03%,FY*B为4.94%,FY*Null为0.03%。19个Fy^a阴性标本信息已登记在册。结论:微流控毛细管电泳系统适用于Duffy血型基因分型筛选,可用于建立稀有血型库,解决稀有血型患者紧急输血问题,提高输血安全。Objective:To explore a method for rapidly screening the Duffy blood group genotypes and to establish an information bank of rare blood type donors.Methods:The microfluidic capillary electrophoresis system and PCR-SSP method were used to analyze the Duffy genotype of 3936 unrelated O-type blood donors in our center from December 2014 to September 2018.The serologic identification and typing of other blood type system phenotypes for Fy^a-negative specimen were performed.The Fy(a-b-)specimen was sequenced for genotyping.Results:The phenotypes of Fy^anegative specimens were consistent with the genotyping results of the microfluidic capillary electrophoresis system.The results of Duffy phenotyping in 3936 specimens were follows:Fy(a+b-)3564(90.54%),Fy(a+b+)353(8.97%),Fy(a-b+)18(0.46%),Fy(a-b-)1(0.03%).The gene frequency was FY*A(95.03%),FY*B(4.94%),and FY*Null(0.03%).The bank of rare blood types of 19 Fy^a-negative specimens was established.Conclusion:Microfluidic capillary electrophoresis system is suitable for Duffy blood group genotyping screening.It can be used to establish a bank of rare blood type,so as to solve the problem of urgent blood transfusion in patients with rare blood type,and to improve blood transfusion safety.

关 键 词：DUFFY血型 芯片电泳 稀有血型库 

分 类 号：R457.1[医药卫生—治疗学]