利用高清晰共聚焦显微镜结合生物发光系统观察体内实时血栓形成  

Observation on the Thrombus Formation in Real Time in Vivo by using High-Resolution Confocal Microscope Combined with Bioluminescence System——Review

作　　者：刘怡晨 陈敏[1] 李东亚 施小凤[1] LIU Yi-Chen;CHEN Min;LI Dong-Ya;SHI Xiao-Feng(Department of Hematology,Jiangsu University Affiliated Hospital,Zhenjiang 212000,Jiangsu Province,China)

机构地区：[1]江苏大学附属医院血液科,江苏镇江212001

出　　处：《中国实验血液学杂志》2020年第5期1787-1790,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金(81700130);江苏省自然科学基金(BK20150474);诺和诺德中国血友病研究基金;镇江市科技创新资金项目(SH2017006);江苏省"科教兴卫"青年医学重点人才基金。

摘　　要：体内血栓形成是一个复杂的过程,除受到血小板、凝血系统、血管影响外,也受到血流的影响。既往检测血栓形成的方法是在体外模拟或静态下完成的,所以不能完全反映体内的血栓形成过程。近几年,新开发的高清晰共聚焦显微镜结合生物发光系统可以直接用在动物体内条件下观察血栓形成。科学家们利用该系统对血栓的形成有了新的认识。本文就该系统的操作过程、利用该系统发现的血栓分层结构、血栓分层结构形成原因及影响因素等作一综述。The process of thrombus formation in vivo is complex,which is affected by the platelets,clotting system,and vessels,as well as blood flow.The previous research ways,which were either static or shear stressmimicking ex vivo,could not reflect the condition in vivo completely.In recent years,The high resolution confocal microscope combined with bioluminescence system was developed which can be used to observe the animal thrombus formation in real time in vivo.By using this system,scientists have gotten a novel knowledge about the thrombus formation.In this review,the operating steps of this system,the hierarchical structure of thrombs revealed by this system and the related mechanism are sammrized briefly.

关 键 词：高清晰共聚焦显微镜 生物发光系统 体内 血栓形成 

分 类 号：R543[医药卫生—心血管疾病] R364[医药卫生—内科学]