棘头梅童鱼中期染色体物理长度的估测  

Physical length of metaphase chromosomes in Collichthys lucidus

作　　者：罗晗娇 刘富江 陈俊男 刘贤德[1] 刘滟溯 蔡明夷 LUO Hanjiao;LIU Fujiang;CHEN Junnan;LIU Xiande;LIU Yansu;CAI Mingyi(Key Laboratory of Healthy Mariculture for the East China Sea,Ministry of Agriculture and Rural Affaris,Fisheries College,Jimei University,Xiamen 361021,China)

机构地区：[1]集美大学水产学院,农业农村部东海海水健康养殖重点实验室,福建厦门361021

出　　处：《水产学报》2020年第11期1824-1831,共8页Journal of Fisheries of China

基　　金：国家自然科学基金(31872553,41706157);集美大学国家级、省级大学生创新创业训练计划(201910390046)。

摘　　要：为了支持棘头梅童鱼基因组学和细胞遗传学研究的发展,本研究采用流式细胞术(flow cytometry,FCM)首次估测了棘头梅童鱼基因组的大小,并利用碘化丙啶染色与图像分析软件测定棘头梅童鱼24对染色体的相对面积、相对长度以及相对累积荧光强度,估算了染色体的物理长度。结果显示,雌性和雄性棘头梅童鱼的基因组大小分别为(802.91±2.19)Mb和(786.52±1.95)Mb。雌性基因组显著大于雄性基因组(约16.39 Mb)。雌性的核型公式为2n=48=48t,臂数NF=48,染色体估测物理长度为(22.17±0.72)^(46.30±1.06)Mb。雄性的核型公式为2n=47=1m+46t,臂数NF=48。其中,雄性特有的m染色体为Y染色体,其估测物理长度为(62.95±2.89)Mb。染色体的估测物理长度与相对面积、相对长度均成正线性相关。研究结果为棘头梅童鱼染色体的识别、配对提供更丰富的信息,为棘头梅童鱼Y染色体的组装及石首鱼科鱼类染色体进化提供参考数据。In order to support the development of genomics and cytogenetics of Collichthys lucidus,in the present study,the genome size of C.lucidus was determined by using flow cytometry,and the relative length,the relative area and the integrated optical density(IOD)of 24 pairs of chromosomes were determined using PI staining and microscopic image analysis software,and the physical length of chromosomes was estimated based on the values of IOD.The results showed that male and female's genome sizes were respectively estimated at(786.52±1.95)Mb and(802.91±2.19)Mb.Female genome was significantly larger than male genome(by about 16.39 Mb).The genome of female comprised 24 pairs of telocentric chromosomes,the karyotype formula is 2n=48=48t,NF=48,with the physical length ranging from(22.17±0.72)Mb to(46.30±1.06)Mb.The genome of male comprised 46 telocentric chromosomes and one metacentric chromosome,the karyotype formula is 2n=47=46t+1m,NF=48.The male specific metacentric chromosome was Y chromosome,with an estimated physical length of(62.95±2.89)Mb.Physical lengths of chromosomes are correlated linearly with the relative area and the relative lengths.In conclusion,new parameters of chromosomes present in this paper will facilitate to chromosome identification and pairing of C.lucidus,which will serve as reference data for the assembly of the Y chromosome and the evolution of chromosome in Sciaenidae.

关 键 词：棘头梅童鱼 基因组大小 染色体物理长度 图像分析 

分 类 号：Q343.2[生物学—遗传学] S917.4[农业科学—水产科学]