2014-2018年广西柳州市HCV的基因型和1b亚型NS5B区的序列特征  

The genotypes and sequence characteristics of NS5B region of subtype 1b among hepatitis C viruses in Liuzhou,Guangxi,from 2014 to 2018

作　　者：尹琦 罗洲[2] 陈钦艳[3] 杨庆利[1] YIN Qi;LUO Zhou;CHEN Qin-yan;YANG Qing-li(Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine,Guangxi University of Chinese Medicine,Nanning 530200,China;Department of Clinical Laboratory,Liuzhou Municipal Liutie Central Hospital,Liuzhou 545007,China;Guangxi Key Laboratory for the Prevention and Control of Viral Hepatitis,Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control,Nanning 530028,China)

机构地区：[1]广西中医药大学广西高发传染病中西医结合转化医学重点实验室,南宁530200 [2]柳州市柳铁中心医院检验科,柳州545007 [3]广西壮族自治区疾病预防控制中心广西病毒性肝炎防治研究重点实验室,广西中医药大学,广西高发传染病中西医结合转化医学重点实验室,南宁530028

出　　处：《中华疾病控制杂志》2020年第10期1228-1232,共5页Chinese Journal of Disease Control & Prevention

摘　　要：目的分析2014―2018年广西柳州市丙型肝炎病毒(hepatitis C virus,HCV)的基因型分布和HCV 1b亚型NS5B区的序列特征。方法收集慢性丙型病毒性肝炎患者血清,提取HCV RNA,反转录后用巢式PCR扩增NS5B区序列,测序后构建进化树分析HCV基因型和基因亲缘关系。用聚类分析法对比HCV 1b本地群、非本地群和参照株NS5B区基因和蛋白序列的差异。结果广西柳州市HCV流行的基因型包括1a、1b、2a/c、3a、3b、6a、6c/d和未分型(NT),分别占3.39%、43.01%、0.42%、13.98%、6.99%、28.81%、3.18%和0.21%;HCV 1b亚型中61.6%的病毒与各参照株进化距离均较远,其比例在2014―2018年分别占HCV 1b亚型的69.8%、78.6%、43.3%、47.6%和59.6%;该群HCV的NS5B基因有15个经常性突变位点,可导致NS5B蛋白I2682V和S2755N发生经常性变异。结论广西柳州市HCV流行的主导基因型依次为1b、6a和3a;HCV 1b亚型中的大部分属于本地亲缘性群,该群HCV的NS5B蛋白存在特征性的变异位点并可能影响直接抗病毒药的作用。Objective To analyze the genotypes distribution of hepatitis C virus(HCV)and the sequence characteristics of NS5 B region of HCV subtype 1 b from 2014 to 2018 in Liuzhou,Guangxi.Methods HCV RNA was extracted from serum of patients with chronic hepatitis C,and the NS5 B gene was amplified by nested PCR after reverse transcription.The genotype and genetic relationship of HCV were analyzed by gene sequencing and constructing gene evolutionary tree.The differences of NS5 B gene and protein sequence among local,non-local and reference strains of HCV 1 b were compared by cluster analysis.Results The genotypes of HCV epidemic in Liuzhou included 1 a,1 b,2 a/c,3 a,3 b,6 a,6 c/d and undermined type(NT),which accounted for 3.39%,43.01%,0.42%,13.98%,6.99%,28.81%,3.18%and 0.21%,respectively.The evolutionary distances for 61.6%of the HCV 1 b subtype were far away from those of the reference strain.From 2014 to 2018,the proportion of this group accounted for 69.8%,78.6%,43.3%,47.6%and 59.6%of HCV 1 b,respectively.Fifteen frequent point mutations of NS5 B gene in this group were determined,which caused I2682 V and S2755 N frequent mutations of NS5 B protein.Conclusions The dominant genotypes of HCV epidemic in Liuzhou,Guangxi are 1 b,6 a and 3 a.Most of the subtypes of HCV 1 b belong to the local genetic group.The NS5 B proteins of this group have characteristic mutational sites and may affect the antiviral effect with direct antiviral drugs.

关 键 词：丙型肝炎病毒 基因型 HCV 1b NS5B区 变异 

分 类 号：R373.2[医药卫生—病原生物学]