人杀菌肽LL-37联合绿原酸对铜绿假单胞菌生物被膜的体外作用  

In vitro effect of human bactericidal peptide LL-37 combined with chlorogenic acid on pseudomonas aeruginosa biofilm

作　　者：刘冬梅 孔晋亮[1] 罗劲[1] 刘丽华[1] 王可[1] 蔡双启[1] 刘唐娟[1] 卢桦崧[1] 解寻 Liu Dongmei;Kong Jinliang;Luo Jing(Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021)

机构地区：[1]广西医科大学第一附属医院呼吸与危重症医学科,广西南宁530021

出　　处：《中国现代医药杂志》2020年第10期1-4,共4页Modern Medicine Journal of China

基　　金：国家自然科学基金资助项目(编号:81760743);广西高校中青年教师科研基础能力提升资助项目(编号:2019KY0125);广西壮族自治区卫生健康委员会自筹经费科研课题资助项目(编号:Z20200825)。

摘　　要：目的通过建立体外铜绿假单胞菌早期生物被膜模型,研究人杀菌肽LL-37联合绿原酸对铜绿假单胞菌生物被膜的作用。方法采用肉汤稀释法测定人杀菌肽LL-37、绿原酸对铜绿假单胞菌浮游菌最低抑菌浓度(MIC),结晶紫定量法测定人杀菌肽LL-37联合绿原酸对铜绿假单胞菌生物被膜的清除作用,荧光显微镜下观察人杀菌肽LL-37、绿原酸及两药联合对铜绿假单胞菌生物被膜结构的作用。结果人杀菌肽LL-37,绿原酸对铜绿假单胞菌浮游菌的MIC分别为256μg/ml、8192μg/ml,联合用药后人杀菌肽LL-37的MIC为64μg/ml,绿原酸的MIC为512μg/ml,FICI值为0.3125。光学显微镜下观察到联合用药组与空白组相比死菌增加,活菌及生物被膜减少。结论LL-37联合绿原酸对抗铜绿假单胞菌以及清除铜绿假单胞菌生物被膜具有协同作用。Objective To investigate the synergetic effect of human bactericidal peptide LL-37 combined with chlorogenic acid on pseudomonas aeruginosa biofilm by establishing early biofilm model of pseudomonas aeruginosa in vitro.Methods The minimal inhibitory concentration(MIC)of human bactericidal peptide LL-37 and chlorogenic acid were determined by broth microdilution method respectively,crystal violet method was used to determine the scavenging effect of human bactericidal peptide LL-37 combined with chlorogenic acid on the removal of pseudomonas aeruginosa biofilm,fluorescence microscope was used to evaluate the biofilm in the presence of these drugs.Results The MIC of human bactericidal peptide LL-37 and chlorogenic acid to pseudomonas aeruginosa planktonic bacteria was 256μg/ml and 8192μg/ml respectively.The MIC of LL-37 combined with chlorogenic acid to pseudomonas aeruginosa planktonic bacteria was 64μg/ml and 512μg/ml,the FICI value was 0.3125.Under the optical microscope,the combined medication group showed that the number of the dead bacteria increased,live bacteria and the biofilm decreased compared with the blank group.Conclusion Human bactericidal peptide LL-37 combined with chlorogenic acid has synergistic effect in combating pseudomonas aeruginosa and removing its biofilm.

关 键 词：人杀菌肽LL-37 绿原酸 铜绿假单胞菌 生物被膜 

分 类 号：R96[医药卫生—药理学]