染料改性胆红素吸附膜的制备  

Preparation of reactive dye-modified membrane for bilirubin adsorption

作　　者：鞠佳 孔鹏飞 封瑞江[1] 梁飞雪 张晓欣 贺高红[2] 杨磊 JU Jia;KONG Pengfei;FENG Ruijiang;LIANG Feixue;ZHANG Xiaoxin;HE Gaohong;YANG Lei(School of Petrochemical Engineering,Liaoning Shihua University,Fushun 113001,Liaoning,China;State Key Laboratory of Fine Chemicals,Department of Chemical Engineering,Dalian University of Technology,Dalian 116024,Liaoning,China)

机构地区：[1]辽宁石油化工大学石油化工学院,辽宁抚顺113001 [2]大连理工大学化工学院,精细化工国家重点实验室,辽宁大连116024

出　　处：《化工进展》2020年第11期4529-4536,共8页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金(21606120,21604034,21527812,U1663223)。

摘　　要：以乙酸纤维素/聚乙烯亚胺共混微滤膜(CA/PEI膜)为基质,通过固定活性染料汽巴蓝F3GA(CBA),制备出一种特异性吸附胆红素的染料改性亲和膜(CA/PEI-CBA膜)。CA/PEI-CBA膜在3300~3500cm^-1处的氨基红外吸收峰较原膜强度增加,在XPS谱图中结合能为167.4eV和167.8eV处新出现S==O和—SO3H的S2p吸收峰,表明CBA分子的成功担载。通过正交实验和单边实验考察了反应温度、反应时间和CBA浓度对染料改性膜的胆红素吸附量和失重率的影响,优化了制备条件。染料改性膜的胆红素吸附量随反应时间的延长与CBA浓度的升高而增大;而随着反应温度的升高,染料改性膜的失重率增加,发现其基质结构因水解而破坏。在反应温度为30℃、CBA浓度选择5mg/mL、反应4h的情况下,制备的染料改性膜保持较好的多孔结构,有利于胆红素与CBA分子的充分接触,表现出良好的吸附性能。In this paper,cellulose acetate/polyethyleneimine blend microfiltration membrane(CA/PEI membrane)was used as the matrix to prepare the dye-modified affinity membrane(CA/PEI-CBA membrane)with specific adsorption for serum bilirubin by immobilizing the reactive dye Cibacron F3GA(CBA).The amino infrared absorption peak of CA/PEI-CBA membrane was stronger at 3300~3500cm-1 than that of the original membrane,and the new S==O and—SO3H S2p absorption peaks of CA/PEI-CBA membrane appeared at the binding energy of 167.4eV and 167.8eV in the XPS spectrum.These results suggested the success of CBA molecules’immobilization.The effects of reaction temperature,reaction time,and the CBA concentration on both bilirubin adsorption capacity and weight loss of dye-modified membrane were investigated and optimized through orthogonal and unilateral experiments.The bilirubin adsorption capacity of dye-modified membrane increased with both reaction time and the CBA concentration.However,with the increase of reaction temperature,the weight loss of the dye-modified membrane increased and the membrane matrix structure was destroyed because of hydrolysis.Under the conditions that reaction temperature was 30℃,CBA concentration was 5mg/mL and reaction time was 4h,the manufactured dye-modified membrane maintained a perfect porous structure,which was conducive to the full contact of bilirubin with CBA molecules and showed a good adsorption performance.

关 键 词：膜 活性染料 优化 吸附 胆红素 

分 类 号：TQ028.8[化学工程]