菠萝SPL基因家族全基因组鉴定及其在开花诱导中的表达分析  

Genome-Wide Identification of SPL Gene Family and Expression Profiling during Flowering Induction in Pineapple

作　　者：潘晓璐 张秀梅[2] 许婷婷 侯世贵 张红娜[2] Pan Xiaolu;Zhang Xiumei;Xu Tingting;Hou Shigui;Zhang Hongna(College of Life Science and Technology,Lingnan Normal University,Zhanjiang,524091;Key Laboratory of Ministry of Agriculture for Tropical Fruit Biology,South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,524091;College of Tropical Crops,Hainan University,Haikou,570102;Chumei Subtropical Fruit Trees Research Institute,Chongqing,402260)

机构地区：[1]岭南师范学院生命科学与技术学院,湛江524091 [2]中国热带农业科学院南亚热带作物研究所,农业部热带果树生物学重点实验室,湛江524091 [3]海南大学热带作物学院,海口570102 [4]重庆市储美果树研究所,重庆402260

出　　处：《分子植物育种》2020年第20期6633-6642,共10页Molecular Plant Breeding

基　　金：国家重点研发计划专项(2018YFD1000504);广东省自然科学基金(2017A030307003);海南省自然科学基金(318MS098);国家自然科学基金青年基金(31601733);国家自然科学基金面上项目(31872079);中央级公益性科研院所基本科研业务费(1630062019013)共同资助。

摘　　要：SQUAMOSA promoter binding-like (SPL)转录因子在植物成花转变和花发育过程中起着重要的调控作用。本研究通过生物信息学方法从菠萝全基因组中共鉴定出16个AcSPL基因,分布于12条染色体上。系统发育分析表明菠萝SPL基因家族可分为5个亚组,相对于双子叶植物拟南芥而言,菠萝的进化关系更接近于单子叶植物(水稻)。基因结构和基序分析发现菠萝SPL基因家族具有相对保守的基因结构和功能结构域。组织特异性表达分析结果显示,8个AcSPLs的最高表达量均出现在花芽中,6个AcSPLs在雌蕊中的表达量高于雄,1个AcSPL在萼片中的表达量最高,表明多数AcSPL基因与菠萝花芽分化及花发育密切相关。AcSPL成员在花芽转录组中的表达结果也暗示SPL家族的不同成员参与乙烯诱导菠萝开花的不同阶段。本研究为菠萝SPL家族重要基因的克隆提供了参考,并为深入了解菠萝SPL基因在成花中的功能提供数据依据。SQUAMOSA promoter binding-like(SPL) transcription factors play important regulatory roles in floral transition and flower development. In this study, 16 AcSPLs distributed in 12 chromosomes were identified from the reference genome of pineapple using bioinformatics. Phylogenetic analysis indicated that AcSPLs were clustered into five groups, the evolutionary relationship of AcSPLs in pineapple was relatively closer with monocotyledonous plant of rice, compared to the dicotyledonous plant of Arabidopsis. AcSPL gene families of pineapple were found have relatively conserved gene structure and functional domain by gene structure and motif analysis. Tissue-specific expressions showed that 8 members of AcSPLs all appeared at the highest expression levels in flower buds, 6 members of AcSPLs appeared the higher expression level in pistil than that in stamen, 1 member of AcSPLs has the highest expression in sepals, suggesting most Ac SPL genes family were closely related to pineapple flower bud differentiation and flower development. Expression results of the floral transcriptome also implied that different AcSPL members may be involved in different floral induction stages of pineapple. This study will provide a reference for the cloning of important genes of SPL family in pineapple, and laid the groundwork for further understanding the flower function of AcSPLs in pineapple.

关 键 词：菠萝(Ananas comosus(L.)Merr.) SPL 全基因组鉴定 成花诱导 

分 类 号：S668.3[农业科学—果树学] Q943.2[农业科学—园艺学]