烟草叶绿体基因组RNA编辑位点的鉴定与分析  

Identification and Analysis of RNA Editing Sites Chloroplast Genome in Nicotiana tabacum

作　　者：王淮 杨健康 Wang Huai;Yang Jiankang(Laboratory of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Dali University,Dali,671000)

机构地区：[1]大理大学基础医学院,生物化学与分子生物学实验室,大理671000

出　　处：《分子植物育种》2020年第20期6649-6656,共8页Molecular Plant Breeding

基　　金：大理大学博士科研启动基金(KYBS201603)资助。

摘　　要：为了更好地了解烟草叶绿体基因组中RNA编辑位点的数量及它们发挥的功能,本研究借助烟草3种器官(叶,花,根)的基因组测序数据及RNA测序数据,对烟草叶绿体基因组里胞嘧啶(C)转换为尿嘧啶(U)的RNA编辑位点进行了鉴定。在烟草叶绿体基因组中,发现了1 002个RNA编辑位点。609个编辑位点位于63个蛋白编码基因,占所有编辑位点的60.8%,其中无义突变的编辑位点46个。蛋白编码基因中,rpoC2、ycf1和ndhD发生的编辑位点最多,RNA基因中trnG、trnfM和trnD有最多的编辑位点。对叶、花和根中的编辑位点进行比较,根的RNA编辑位点最多,为652个。这些无义突变的RNA编辑位点,在花、根中主要是影响参与光合作用的基因,而在叶中这些基因未受影响。表明RNA编辑作为转录后调控的一种方式,对于维持不同器官的功能是非常重要的。本研究为进一步研究烟草RNA编辑的生物学功能提供帮助。In order to better understand the number of RNA editing sites and their function in the chloroplast genome of tobacco, the RNA editing sites of cytosine(C) converted to uracil(U) in chloroplast genome were identified in this study by genome and RNA sequencing data of three organs of tobacco, including leave, flower and root. In the chloroplast genome of tobacco, 1 002 RNA editing sites were found. Among them, 609 editing sites were located in 63 protein-coding genes, accounting for 60.8% of all editing sites, including 46 editing sites for nonsense mutations. Among the protein-coding genes, rpoC2, ycf1 and ndhD had the most editing sites, while trnG, trnfM and trnD showed the most editing sites in the RNA gene. Comparing the number of editing sites in leave, flower and root, there are 652 RNA editing sites were found in root, which were the most in the three organs. The RNA editing sites of these nonsense mutations mainly affect the genes involved in photosynthesis in flower and root,while these genes are not affected in leave. It can be seen that RNA editing as one of the mechanisms of post-transcriptional regulation is very important for maintaining the functions of different organs. This study will be helpful to further research on the biological functions of RNA editing of tobacco.

关 键 词：烟草(Nicotiana tabacum) 叶绿体 RNA编辑 二代测序 

分 类 号：S572[农业科学—烟草工业] Q943.2[农业科学—作物学]