巴西橡胶树GolS和RS家族基因理化特性与表达特征分析  

Characterization and Expression Profiles of Galactinol Synthase and Raffinose Synthase in Rubber Tree

作　　者：卢基来 王志勇[1] 龙翔宇[2] 方永军[2] 周明旭 阳江华[2] 秦云霞[2] Lu Jilai;Wang Zhiyong;Long Xiangyu;Fang Yongjun;Zhou Mingxu;Yang Jianghua;Qin Yunxia(College of Tropical Crop,Hainan University,Haikou,570228;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101;College of Tropical Crop Science,Yunnan Agricultural University,Puer,665000)

机构地区：[1]海南大学热带作物学院,海口570228 [2]中国热带农业科学院橡胶研究所,海口571101 [3]云南农业大学热带作物学院,普洱665000

出　　处：《分子植物育种》2020年第20期6665-6670,共6页Molecular Plant Breeding

基　　金：海南省自然科学基金(2019CR336);国家自然科学基金面上项目(31770709,31971680);中央级公益性科研院所基本科研业务费专项(1630022017003;1630022019016)共同资助。

摘　　要：肌醇半乳糖苷合成酶(galactinol synthase, GolS)和棉子糖合成酶(raffinose synthase, RS)是棉子糖系列寡糖(raffinose family oligosaccharides, RFOs)代谢的重要酶,广泛参与植物的生长发育以及逆境胁迫抗性等。本研究基于橡胶树基因组数据库平台,分离鉴定Gol S和RS家族成员,重点分析其表达模式,以了解其在橡胶树叶片发育及割胶过程中的作用。结果表明:HbGolS1和HbRS1分别是乳管中两家族的关键成员,HbGolS1在叶片和胶乳中高表达,随叶片发育其丰度逐渐升高,在成熟叶片中达到峰值。此外,该基因受割胶显著诱导上调表达,乙烯利明显抑制其表达;与之不同,HbRS1在胶乳中特异表达,不受割胶和乙烯利处理的影响。本研究结果阐明了HbGolS和HbRS随叶片发育及割胶的表达特性,为进一步解析橡胶树中RFOs代谢及其功能依据。Galactinol synthase(GolS) and raffinose synthase(RS) are key enzymes for the synthesis of raffinose family oligosaccharides(RFOs) which are widely involved in the growth development and resistances to stresses.To explore the function of HbGolSs and HbRSs during leaf development and tapping period in Hevea brasiliensis.In this study, based on the established library data platform of rubber tree genome, we isolated and identified the family members of HbGolSs and HbRSs, then we analyzed their expression profiles with emphasis through on-line molecular biological tools and PRISM software as well. The results demonstrated that HbGolS1 and HbRS1 were prime members in their separate family, HbGolS1 was highly expressed in latex and leaf, and with leaf development, its level gradually increased till the ultimate in mature leafve, especially, It was dramatically up-regulated by tapping but markedly inhibited by ethrel treatment;different from it, HbRS1 was little influenced by tapping or ethrel treatment although it was specially expressed in latex. Together, this study demonstrated the expression characteristics of HbGolS1 and HbRS1 with leaf development and tapping, which has laid a foundation for further exploring the metabolism of RFOs and their roles in rubber trees.

关 键 词：逆境胁迫 半乳糖醇合成酶 棉子糖合成酶 

分 类 号：S794.1[农业科学—林木遗传育种] Q943.2[农业科学—林学]