萝卜抽薹进程中生理生化变化及开花基因时空表达比较  

Physiological and Biochemical Changes and Spatial and Temporal Expressions of Flowering Genes in Radish(Raphanus sativus L.) during Bolting

作　　者：何磊 姚南 裴芸[1] 俞甜 祖贵东 张万萍[1] He Lei;Yao Nan;Pei Yun;Yu Tian;Zu Guidong;Zhang Wanping(School of Agriculture,Guizhou University,Guiyang,550025;Xingshi Town Agriculture,Forestry and Water Integrative Service Center,Longli County,Qiannan Prefecture,Longli,551206;Bijie Weining County Agricultural Bureau,Weining,553100)

机构地区：[1]贵州大学农学院,贵阳550025 [2]黔南州龙里县醒狮镇农林水综合服务中心,龙里551206 [3]毕节市威宁县农业局,威宁553100

出　　处：《分子植物育种》2020年第20期6837-6843,共7页Molecular Plant Breeding

基　　金：国家自然科学基金(31960598);贵州省科技计划项目(黔科合支撑[2019]2256)共同资助。

摘　　要：为了解析萝卜抽薹的生理生化和分子机理,以期为萝卜耐抽薹资源的鉴定和利用提供基础,以一对抽薹特性差异显著的萝卜品种‘韩国大根’(极耐抽薹)和‘新西兰短叶13号’(极易抽薹)为研究材料,分别于花芽未分化期、花芽分化期、现蕾期、薹高2~5 cm和薹高>5 cm时期测定了萝卜叶片中过氧化氢酶(CAT)活性、过氧化物酶(POD)活性、可溶性糖含量和可溶性蛋白含量等指标,并对花芽未分化期、花芽分化期及现蕾期等时期萝卜根、叶和茎尖3个部位的开花相关基因的时空表达水平进行了分析。结果表明,在萝卜抽薹进程中,可溶性糖含量、可溶性蛋白含量、SOD、POD和CAT等酶活性等呈趋势性变化;FLC1.1和FLC1.2在耐抽薹萝卜材料中表达量均显著高于易抽薹材料,VRN1、VRN2及SOC1则在易抽薹材料中含量显著高于耐抽薹材料。研究结果为了解萝卜抽薹的生理生化和分子基础、花芽分化和抽薹的鉴定以及耐抽薹萝卜种质资源的挖掘提供理论基础。In order to explore the physiological and molecular mechanisms underlying the bolting of Raphanus sativus L., thus to provide the basis for the identification and utilization of bolting resistance resources of radish. A pair of radish varieties ’Korea Dagen’(extremely bolting tolerant) and ’New Zealand Short Leaf 13’(extremely bolting sensitive) were used in this study. Physiological indexes such as catalase(cat) activity, peroxidase(POD)activity, soluble sugar content, soluble protein content and other physiological indexes in radish leaves were measured at five different stages, including non-flower bud stage, flower bud differentiation stage, flowering stage,bolting stage with 2~5 cm bolting height and bolting stage with >5 cm bolting height. The spatial-temporal expression patter of flowering related genes were analyzed at non-flower bud stage, flower bud differentiation stage and flowering stage. The results showed that the soluble sugar content, soluble protein content, SOD, POD, CAT and other enzyme activities showed a trend change during the bolting process of radish. FLC1.1 and FLC1.2 expression levels in the bolting-resistant radish variety are significantly higher than those in the bolting-sensitive variety,whereas expression levels of VRN1, VRN2 and SOC1 in the bolting-sensitive variety are significantly higher than that in the bolting-tolerant variety. The results will help to understand the physiological and molecular mechanism of radish bolting and provide theoretical basis for the identification of flower bud differentiation and bolting and exploring bolting resistant radish germplasm resources.

关 键 词：萝卜(Raphanus sativus L.) 抽薹 生理指标 开花基因 时空表达分析 

分 类 号：S631.1[农业科学—蔬菜学]