电子烟雾化剂丙三醇90天大鼠吸入毒性研究  

Inhalation toxicity testing on Wistar rats exposed to atomized glycerol in 90 days

作　　者：严大为 高峄涵 盛云华[2] 梁德民 胡玥[2] 郑赛晶 YAN Dawei;GAO Yihan;SHENG Yunhua;LIANG Demin;HU Yue;ZHENG Saijing(Department of Basic Research,Shanghai New Tobacco Product Research Institute,Shanghai 201315,China;Department of Pharmacology and Toxicology,Shanghai Institute for Food and Drug Control,Shanghai 201203,China;Technical Center of Shanghai Tobacco Group CO.,Ltd.,Shanghai 201315,China)

机构地区：[1]上海新型烟草制品研究院基础研究室,201315 [2]上海市食品药品检验所药理毒理室,201203 [3]上海烟草集团有限责任公司技术中心,201315

出　　处：《中国烟草学报》2020年第5期1-9,共9页Acta Tabacaria Sinica

摘　　要：【目的】了解电子烟雾化剂丙三醇的吸入毒性。【方法】选择120只Wistar大鼠进行90天吸入毒性试验(恢复期28天)。按照大鼠体重随机分为阴性对照组、低剂量组(10 mg/kg)、中剂量组(100 mg/kg)和高剂量组(750 mg/kg),每组动物30只,雌雄各半。试验期间检测大鼠体重和耗食量变化;在染毒期和恢复期结束后,解剖大鼠进行血液学、血生化、尿液等指标检测和肺部支气管灌洗液分析,并对大鼠脏器进行组织病理学检查。【结果】与阴性对照组相比,①体重与耗食量:中剂量组雄性大鼠第3周耗食量降低(P<0.05),高剂量组雌性大鼠第6和11周耗食量降低(P<0.05);②血液学:恢复期中剂量组雄性大鼠,MCH(平均红细胞血红蛋白)水平上升(P<0.05);PLT(血小板数)水平下降(P<0.05);③血生化:处理组的各项指标均未见显著性差异(P>0.05)④尿液:恢复期低剂量组雄性大鼠的尿比重升高(P<0.05),⑤脏器重量及系数:染毒期高剂量组雌性大鼠肝脏重量、肾脏重量均低于阴性对照组(P<0.0);恢复期中剂量组雄性大鼠肺重量低于阴性对照组(P<0.01),⑥肺部支气管灌洗液分析:处理组的各项指标均未见显著性差异(P>0.05)⑦组织病理学检查:阴性对照组和高剂量组中大鼠的脏器组织未见明显病变。经分析以上的指标变化未呈现量效关系。【结论】大鼠鼻吸入丙三醇90天,暴露剂量750 mg/kg情况下未对大鼠产生明显的毒性作用。[Objective]This work aims to understand the inhalation toxicity of glycerin as electronic cigarette atomizing agent.[Methods]In this work,120 Wistar rats were selected as research objected subjected to 90 days of inhalation toxicity test,with 28 days of recovery.The rats were randomly divided into a negative control group,a low-dose group(10 mg/kg),a medium-dose group(100 mg/kg)and a high-dose group(750 mg/kg)according to their body weight.There were 30 animals in each group,half male and half female.During the experiment,the changes in animal body weight and food consumption were detected.After the exposing period and recovery period,hematology,blood biochemistry,urine and other endpoints were detected,Broncho alveolar lavage fluid was analyzed,and histopathological evaluation of animal organs was conducted.[Results]Compared with the negative control group,①The male rats in the medium dose group exhibited significantly decreased food consumption at week 3(P<0.05),while the female rats in the high dose group exhibited significantly decreased food consumption at week 6 and week 11(P<0.05).②The MCH(mean erythrocyte hemoglobin)level significantly increased in the male rats of medium dose group during recovery(P<0.05)and the level of PLT(platelet count)was significantly decreased(P<0.05).③There was no significant difference in blood biochemistry among the treatment groups(P>0.05).④The urine specific gravity in the low dose group increased during recovery period(P<0.05).⑤The weight of liver and kidney in female rats of the high-dose group were lower than that of the negative control group during exposing period(P<0.01).During the recovery period,lung weight in male rats of the medium dose group was lower than that of the negative control group(P<0.01).⑥There was no significant difference in broncho alveolar lavage fluid:among the treatment groups(P>0.05).⑦No obvious lesions were found in the organs of the negative control group and the high-dose group according to histopathological evaluation.The changes

关 键 词：电子烟 丙三醇 大鼠 吸入毒性 

分 类 号：R994.1[医药卫生—毒理学]