基于中药系统鉴定法进行青葙子药材的鉴别研究  

Identification of Celosia argentea based on Systematic Identification of Traditional Chinese Medicine

作　　者：谭新宁 吴文如[1] 来慧丽[2] 张戴英 安鑫 黄玉桦 陈镇舟 TAN Xin-ning;WU Wen-ru;LAI Hui-li;ZHANG Dai-ying;AN Xin;HUANG Yu-hua;CHEN Zhen-zhou(Traditional Chinese Medicine College,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Guangdong Food and Drug Vocational College,Guangzhou 510006,China;Guangdong Hexiang Pharmaceutical Co.Ltd.,Guangzhou 510006,China)

机构地区：[1]广州中医药大学中药学院,广州广东510006 [2]广东食品药品职业学院,广州广东510006 [3]广东和翔制药有限公司,广州广东510006

出　　处：《中药材》2020年第2期296-302,共7页Journal of Chinese Medicinal Materials

基　　金：国家中医药管理局全国中药资源普查项目(GZY-KJS-2018-004);全国中医药创新骨干人才培训项目(国中医药人教函[2019]128号);广州中医药大学大学生创新创业训练计划项目(201910572161)。

摘　　要：目的:利用中药系统鉴定法鉴别青葙子及其混伪品,为青葙子药材的准确鉴定及质量控制提供参考。方法:通过性状、微性状、显微鉴别和DNA条形码分析等方法,对青葙子药材及其混伪品进行比对分析,利用MEGA 7.0软件计算物种DNA条形码序列的种内、种间Kimura 2-Parameter遗传距离、评估序列的条形码间距,采用邻接法构建系统聚类树。结果:仅凭性状鉴别难以区分青葙子与混伪品;青葙子及其混伪品的微性状、显微特征差异明显,可作为其鉴定的重要依据之一;经筛选,推荐ITS序列作为青葙子及其混伪品的DNA条形码候选序列,trnL序列作为补充序列。结论:中药系统鉴定法能够完成对青葙子药材的精准鉴别。Objective:To identify Celosia argentea and its adulterants by systematic identification of traditional Chinese medicine,in order to provide reference for the accurate identification and quality control of Celosia argentea.Methods:Through the methods of characterization,microcharacterization,microscopic identification and DNA barcoding analysis,the comparative analysis of Celosia argentea and its adulterants was carried out.MEGA 7.0 software was used to calculate the genetic distance of in-species and inter-species Kimura 2-Parameter of DNA barcoding sequences of species and evaluate the barcoding gap of sequences,the system cluster tree was constructed by neighbor-joining method.Results:It was difficult to distinguish Celosia argentea and its adulterants only by character identification.The differences in microcharacters and microscopic characteristics of Celosia argentea and its adulterants were obvious,which could be used as an important basis for identification.After screening,the ITS sequence was recommended as the candidate sequence of DNA barcoding of Celosia argentea and its adulterants,and the trnL sequence was used as the supplementary sequence.Conculsion:The systematic identification of traditional Chinese medicine can be used for accurate identification of Celosia argentea.

关 键 词：青葙子 性状鉴别 微性状鉴别 显微鉴别 DNA条形码 

分 类 号：R282.71[医药卫生—中药学]