我国部分省域甘蓝主要产区黑腐病菌鉴定及致病力分析  

Identification and analysis of pathogenicity for black rot strains in central cabbage productive areas of some provinces in China

作　　者：张鑫鑫 张恩慧[1] 吴云锋[2] 蔡美杰 孙庆国 李岸 王谦 ZHANG Xin-xin;ZHANG En-hui;WU Yun-feng;CAI Mei-jie;SUN Qing-guo;LI An;WANG Qian(State Key Laboratory of Crop Stress Biology for Arid Areas/College of Horticulture,Northwest A&F University,Yangling 712100,China;College of Plant Protection,Northwest A&F University,Yangling 712100,China)

机构地区：[1]旱区作物逆境生物学国家重点实验室/西北农林科技大学园艺学院,杨凌712100 [2]西北农林科技大学植保学院,杨凌712100

出　　处：《植物病理学报》2020年第5期592-601,共10页Acta Phytopathologica Sinica

基　　金：国家“十三五”重点研发计划项目(2017YFD0101804)。

摘　　要：为了明确我国甘蓝主栽区黑腐病菌株种类与致病力水平,为优化抗病性鉴定方法、筛选抗病品种及抗病品种的合理布局提供依据,分别采集了10个省域的黑腐病病叶,经过分离纯化与分子鉴定,获得了26个典型甘蓝黑腐病菌株;选用5个甘蓝抗、感品种作为鉴别寄主,以甘蓝黑腐病菌株的悬浮液为接种体,采用喷雾接种法测定致病力。结果表明:来源于不同省域的黑腐病菌株致病力存在显著差异,致病力最强的是YU,病情指数平均值为38.31;致病力最弱的是SH,病情指数平均值为17.03。来源于不同海拔高度的甘蓝黑腐病菌株致病力最强的是G1500,病情指数平均值为37.93;最弱的是G800,病情指数平均值为19.80。根据鉴别寄主抗感反应将26个黑腐病菌株划分为12类致病型,发现来自不同地域的甘蓝黑腐病菌株间存在着显著的致病性分化现象,对甘蓝品种的致病力存在着显著性差异,其中菌株YU致病力最强,菌株YU可作为甘蓝抗病性鉴定的主要致病菌株,指导抗病品种的选育。In order to clarify the species and pathogenicity of black rot strains in central cabbage productive areas of China,and provide a basis for optimizing the identification methods of disease resistance,screening resistant varieties and rational distribution of resistant varieties,black rot disease leaves were collected from 10 provinces.After isolation,purification and molecular identification,26 typical strains from central cabbage productive areas in 10 different provinces were obtained.5 cabbage resistant and susceptible varieties were selected as the test hosts.The suspension of the black rot strain was an inoculum and the pathogenicity was determined by a spray inoculation method.The results showed that there were significant differences in the pathogenicity of black rot strains from different provinces.The most pathogenic strainis YU and the average disease index is 38.31.The weakest strain is SH and the disease index is 17.03.The most virulent cabbage black rot strain from different altitudes is G1500,the average disease index is 37.93;the weakest is G800,the average disease index is 19.80.According to the identification of host anti-infective reaction,26 black rot strains can be preliminarily classified into 12 types of pathogenic types.It was found that there were significant pathogenic differentiation among cabbage black rot strains from different regions,and there were significant differences in the pathogenicity to cabbage varieties.Among them,strain YU led to strongest pathogenicity,and can be used as the main pathogenic strain for the identification of disease resistance of cabbage,which guides the breeding of resistant varieties.

关 键 词：甘蓝 黑腐病菌 病情指数 致病力差异 

分 类 号：S635[农业科学—蔬菜学] S436.35[农业科学—园艺学]