小麦纹枯病菌Rc-RPA-LFD快速检测方法的建立及应用  

Development and application of Rc-RPA-LFD for the rapid detection of Rhizoctonia cerealis

作　　者：鞠玉亮 沈鹏飞 冯艳娟 羊国根 刘文成 郭文华 潘月敏[1] JU Yu-liang;SHEN Peng-fei;FENG Yan-juan;YANG Guo-gen;LIU Wen-cheng;GUO Wen-hua;PAN Yue-min(Key Laboratory of Biology and Sustainable Management of Plant Disease and Pests of Anhui Higher Education Institutes,Innovation Team of Green Pesticide Development and Application in Anhui Province,Anhui Agricultural University,Hefei 230036,China;Agricultural Technology Promotion Center of Yongqiao District,Suzhou 234000,China)

机构地区：[1]安徽农业大学植物保护学院,植物病虫害生物学与绿色防控安徽普通高校重点实验室,安徽省绿色农药研发与应用创新团队,合肥230036 [2]宿州市埇桥区农业技术推广中心,宿州234000

出　　处：《植物病理学报》2020年第5期618-621,共4页Acta Phytopathologica Sinica

基　　金：国家重点研发计划子课题(2017YFD2021708);安徽省科技重大专项(17030701050)。

摘　　要：近年来,随着气候条件、耕作制度的变化和水肥条件的改善,小麦纹枯病在我国危害日趋严重,现已成为长江中下游麦区和黄淮麦区主要病害,每年造成严重经济损失[1]。小麦纹枯病常与小麦根茎部病害如根腐病、茎基腐病混合发生,发病早期症状相似,影响病害的早期诊断和防治。Rhizoctonia cerealis is a soil-borne phytopathogenic fungus that causes wheat sharp eyespot,resulting in serious economic losses.In this study,the recombinase polymerase amplification combined with lateral-flow dipstick technology(Rc-RPA-LFD)was developed for the rapid and sensitivity detection of R.cerealis.The Rc-RPA-LFD assay could be completed at isothermal temperature of 38°C within 30 min without PCR thermal cyclers.The RPA primers and probe designed based on ITS region,showed high specificity to R.cerealis.The detection limit of Rc-RPA-LFD assay was 1 pg·μL-1 fungal genomic DNA,showed an equal sensitivity to that of conventional PCR.In addition,the Rc-RPA-LFD assay could detect R.cerealis from field soil samples,showing no significant differences compared to conventional PCR assay.The simplicity,rapidly and practicability all indicated that Rc-RPA-LFD assay will be a promising molecular diagnosis for the accurate and rapid detection of R.cerealis.

关 键 词：小麦纹枯病 水肥条件 茎基腐病 黄淮麦区 根腐病 诊断和防治 耕作制度 快速检测方法 

分 类 号：S432.44[农业科学—植物病理学]