海南岛西瓜病毒病种类鉴定及发生分布  

Distribution and identification of watermelon viruses in Hainan island

作　　者：车海彦[1] 曹学仁[1] 贺延恒 罗大全[1] CHE Hai-yan;CAO Xue-ren;HE Yan-heng;LUO Da-quan(Environment and Plant Protection Institute,CATAS,Haikou 571101,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海口571101

出　　处：《植物病理学报》2020年第5期632-636,共5页Acta Phytopathologica Sinica

基　　金：2018年海南省重大科技计划项目(zdkj201813);中国热带农业科学院基本业务费专项项目(19CXTD-33)。

摘　　要：病毒病是西瓜生产上的重要病害,目前侵染我国西瓜的病毒主要有西瓜花叶病毒(watermelon mosaic virus,WMV)、黄瓜花叶病毒(cucumber mosaic virus,CMV)、黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)、甜瓜黄斑病毒(melon yellow spot virus,MYSV).小西葫芦黄花叶病毒(zucchini yellow mosaic virus,ZYMV)、烟草花叶病毒(tobacco mosaic virus,TMV)、甜瓜酚传黄化病毒(melon aphid-borne yellows virus,MABYV)、番木瓜环斑病毒(papaya ring spot virus,PRSV)。From October 2017 to April 2019,a total of 290 suspected virus-infected watermelon leaf samples were collected in 7 urban areas(Wenchang,Wanning,Lingshui,Sanya,Ledong,Dongfang,Changjiang)in Hainan island.Nine viruses were detected including MYSV,WSMoV,CCYV,CMV,TMV,CGMMV,ZYMV,WMV and PRSV by small RNA deep sequencing and RT-PCR.The detection rates of MYSV,CCYV,CGMMV,ZYMV,CMV,WSMoV,PRSV,TMV and WMV were 66.90%,25.86%,22.07%,17.59%,10.00%,7.59%,5.52%,2.76%and 1.03%respectively.The co-infection rate of virus was 56.56%and 14 types of mixed infections were detected.The results of this study clarify the identification and distribution of waterme-lon-infecting viruses in Hainan.

关 键 词：黄瓜绿斑驳花叶病毒 番木瓜环斑病毒 烟草花叶病毒 黄瓜花叶病毒 西瓜花叶病毒 西瓜生产 种类鉴定 yellow 

分 类 号：S432.41[农业科学—植物病理学]