利用酵母双杂交膜系统筛选与小麦蓝矮植原体免疫膜蛋白互作的异沙叶蝉蛋白  

Screening of leafhopper(Psammotettix alienus L.)proteins interacting with immunomembrane protein of Wheat blue dwarf phytoplasma using yeast two-hybrid membrane system

作　　者：陆文静[1,2] 周通[2] 王桂玲[2] 宋成艳[2] 鄂文顺[2] 周雪松[2] 丁磊[1] 吴云锋 LU Wen-jing;ZHOU Tong;WANG Gui-ling;SONG Cheng-yan;E Wen-shun;ZHOU Xue-song;DING Lei;WU Yun-feng(State Key Laboratory of Crop Stress Biology for Arid Areas,Key Laboratory of Crop Pest Integrated Pest Management on Crop in Northwestern Loess Plateau,Ministry of Agriculture,College of Plant Protection,Northwest A&F University,Yangling 712100,China;Rice Research Institute of Heilongjiang Academy of Agricultural Sciences,Jiamusi 154026,China)

机构地区：[1]旱区作物逆境生物学国家重点实验室,农业部西北黄土高原作物有害生物综合治理重点实验室,西北农林科技大学植物保护学院,杨凌712100 [2]黑龙江省农业科学院水稻研究所,佳木斯154026

出　　处：《植物病理学报》2020年第5期637-640,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(31570144);高等学校学科创新引智计划项目(B07049)。

摘　　要：小麦蓝矮病(Wheat blue dwarf,WBD)是我国西北麦区一种重要植原体病害,在我国西部地区危害严重。该病害由异沙叶蝉(Psammotettix alienus L.)专化性传播,介体传毒成为病害流行的重要中心环节[1]。本实验室前期通过免疫荧光标记研究发现WBD植原体免疫膜蛋白(immunodominant membrane protein,IMP)与介体异沙叶蝉肌动蛋白互作,说明IMP在植原体传播和致病过程中起关键作用。In order to investigate interactive proteins in leafhopper(Psammotettix alienus L.) with WBD phytoplasma, the protein interaction analysis was performed by using WBD phytoplasma IMP(immunodominant membrane protein) as bait protein to screen a cDNA library of leafhopper using a split-ubiquitin yeast membrane system. Through the screening test, 30 clones were obtained from the cDNA library and 8 proteins were identified by searching against NCBI database, such as tubulin, peptidyl-prolyl cis-trans isomerase, Cdc42 protein, ribosomal proteins and ATP-F0 subunit protein, etc. The interactions between IMP and 8 putative proteins were further confirmed by co-transformation and β-Galactosidase assays. This study could be useful for understanding the molecular interaction mechanism between WBD phytoplasma and insect vector and the specificity of transmission.

关 键 词：植原体 病害流行 致病过程 专化性 免疫荧光标记 传毒 酵母双杂交 介体 

分 类 号：S432.41[农业科学—植物病理学]