对代谢综合征痰证和脂肪含量与肥胖相关基因相关性的研究  

Correlation between phlegm pattern of metabolic syndrome,fat mass and obesity-associated(FTO)gene

作　　者：李缘缘 张萍[1,2] 许超强 李丹 陈淑娇[3,4] 高碧珍 Li Yuanyuan;Zhang Ping;Xu Chaoqiang;Li Dan;Chen Shujiao;Gao Bizhen(Fujian University of Traditional Chinese Medicine,Fujian 350122,China;Chinese Medicine Health Management 2011 Collaborative Innovation Center in Fujian Province,Fujian 350122,China;Fujian Key Laboratory of TCM HeaUh Status,Fujian 350122,China;Third People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine,Fujian 350122,China;Key Laboratory of TCM Syndrome Research in Fujian Province University,Fujian 350122,China)

机构地区：[1]福建中医药大学,福建350122 [2]福建省2011中医健康管理协同创新中心 [3]福建省中医健康状态辨识重点实验室 [4]福建中医药大学附属第三人民医院 [5]中医证研究福建省高校重点实验室

出　　处：《北京中医药大学学报》2020年第8期689-695,共7页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.81873234,No.81273666);福建省自然科学基金面上项目(No.2018J01875);福建省2011中医健康管理协同创新中心资助项目(No.JG2017004-协同)。

摘　　要：目的分析代谢综合征(MS)痰证、非痰证患者外周血中脂肪含量与肥胖相关(FTO)基因表达情况及其与痰证素、理化指标之间的相关性,初步探索MS痰证的遗传学基础。方法采用"证素辨证法"将MS患者分为痰证组和非痰证组,各35例,同时以35例正常人为对照。采集外周静脉血行生化指标检测,并以实时荧光定量PCR和酶联免疫吸附测定(ELISA)法分别检测全血FTO mRNA和血清FTO蛋白的表达量。结果正常组、MS痰证组、MS非痰证组FTO mRNA及蛋白相对表达量差异有统计学意义(P<0.05),FTO mRNA表达量由高到低依次为MS痰证组、MS非痰证组、正常组,蛋白表达量由高到低依次为正常组、MS非痰证组、MS痰证组。MS痰证组尿酸(UA)、体重指数(BMI)与痰证素积分正相关(P<0.05),MS非痰证组谷丙转氨酶(ALT)与痰证素积分正相关(P<0.01)。MS痰证组FTO mRNA表达量与低密度脂蛋白(LDL-C)正相关(P<0.05),FTO蛋白表达量与LDL-C负相关(P<0.05);MS非痰证组FTO mRNA表达量与谷草转氨酶(AST)正相关(P<0.01),与尿素氮(BUN)负相关(P<0.05)。结论FTO mRNA的高表达可能使MS痰证形成的风险增高,MS痰证组外周血UA、BMI、LDL-C水平,MS非痰证组外周血ALT、AST、BUN水平可能与FTO基因的高表达有关,提示FTO基因可能作为MS痰证诊断的辅助指标。Objective To analyze the expression of fat mass and obesity-associated(FTO)genes in the peripheral blood of patients with phlegm pattern of metabolic syndrome(MS)and non-phlegm syndrome,and its correlation with elements and physicochemical index of phlegm pattern,so as to explore the genetic basis of MS phlegm pattern,and provide microcosmic index for the TCM diagnosis of MS phlegm pattern.Methods According to the method of"element-based pattern differentiation",MS patients were divided into phlegm pattern group and non-phlegm pattern group(n=35),and 35 healthy people were recruited as the control group.The peripheral venous blood was collected for biochemical examination,and the expression of FTO gene mRNA and protein in human whole blood and serum were detected by real-time fluorescence quantitative PCR(probe method)and enzyme-linked immunosorbent assay(ELISA)method respectively.Results①The relative expressions of FTO mRNA from high to low were phlegm pattern group>non-phlegm pattern group>control group,and the difference between the three groups was statistically significant(P<0.05);②The expressions of FTO protein from high to low were control group>non-phlegm pattern group>phlegm pattern group,and the difference between the three groups was statistically significant(P<0.05);③The uric acid(UA)and BMI in the phlegm syndrome group were positively correlated with the score of phlegm pattern elements(P<0.05,P<0.01),and the alanine transaminase(ALT)in the non-phlegm pattern group was positively correlated with the score of phlegm pattern elements(P<0.01);④FTO mRNA expression was positively correlated with low-density lipoprotein(LDL-C)in the phlegm pattern group(P<0.05),positively correlated with aspartate aminotransferase(AST)in the non-phlegm pattern group(P<0.01).FTO mRNA expression was negatively correlated with Urea nitrogen(BUN)in the non-phlegm pattern group(P<0.05),and negatively correlated with LDL-C in the phlegm pattern group(P<0.05).Conclusion The high expression of FTO gene mRNA may increase th

关 键 词：代谢综合征 痰证 脂肪含量与肥胖相关基因 证素 理化指标 

分 类 号：R241.9[医药卫生—中医诊断学]