电针干预放射性脑损伤小鼠海马区突触可塑性相关蛋白的表达  

Electroacupuncture intervention on the expression of synaptic plasticity-related proteins in the hippocampi of mice with radiation-induced brain injury

作　　者：王冬慧 武鑫[1] 孙宁宁 张晗 高剑峰[2] Wang Donghui;Wu Xin;Sun Ningning;Zhang Han;Gao Jianfeng(School of Basic Medicine,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,Henan Province,China;Graduate School of Henan University of Traditional Chinese Medicine,Zhengzhou 450046,Henan Province,China)

机构地区：[1]河南中医药大学基础医学院,河南省郑州市450046 [2]河南中医药大学研究生院,河南省郑州市450046

出　　处：《中国组织工程研究》2021年第14期2205-2210,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81373852),项目负责人:高剑峰。

摘　　要：背景:放射性脑损伤作为放疗后的严重并发症之一,严重危害人们的健康,损害学习及记忆功能,但是关于电针干预防治放射性脑损伤的报道相对较少。目的:探讨电针干预对放射性脑损伤小鼠突触可塑性相关蛋白表达的影响。方法:将30日龄C57BL/6J小鼠随机分为空白组、模型组和电针组。除空白组外,其余各组给予8 Gy放射剂量构建放射性脑损伤模型,电针组给予针刺"百会""风府"及双侧"肾俞"穴干预21 d,同时腹腔注射BrdU。电针结束后,采用Morris水迷宫实验和T迷宫实验检测小鼠学习记忆能力,免疫组化法检测海马区BrdU阳性表达,Western blot检测海马区Notch信号通路相关蛋白Notch1和Hes1以及突触可塑性相关蛋白突触素、突触后致密蛋白95、脑源性神经营养因子的表达。结果与结论:①电针干预显著改善放射性脑损伤小鼠学习记忆障碍;②模型组BrdU阳性表达较空白组显著减少(P <0.01),电针组BrdU阳性表达较模型组显著增加(P <0.01);③模型组Notch1、Hes1、突触后致密蛋白95、突触素和脑源性神经营养因子表达量较空白组降低(P <0.01,P <0.01,P <0.01,P <0.05,P <0.01);与模型组比较,电针组Notch1、突触后致密蛋白95、突触素和脑源性神经营养因子表达量增加(P <0.01,P <0.01,P <0.05,P <0.01),Hes1表达量明显降低(P <0.05);④结果表明,电针干预改善放射性脑损伤小鼠学习记忆功能的机制可能与Notch信号通路以及电针调节突触可塑性蛋白表达增加有关。BACKGROUND:Radiation-induced brain injury,as one of the serious complications after radiotherapy,seriously endangers people’s health and damages learning and memory functions.However,there are relatively few reports on the prevention and treatment of brain damage caused by electroacupuncture intervention.OBJECTIVE:To explore the effect of electroacupuncture intervention on the expression of synaptic plasticity related proteins in mice with radiation-induced brain injury.METHODS:C57 BL/6 J mice,30 days old.were randomly divided into blank group,model group and electroacupuncture group.Except for the blank group,the other groups were given 8 Gy radiation dose to construct a radiation-induced brain injury model.The electroacupuncture group was given acupuncture at"Baihui"(GV 20),"Fengfu"(GV 16)and bilateral"Shenshu"(BL 23)for 21 days for intervention,and mice were intraperitoneally injected with BrdU.After electroacupuncture,Morris water maze test and T maze test were used to detect the learning and memory abilities of mice.Immunohistochemical method was used to detect the expression of BrdU in the hippocampus.Western blot assay was used to detect Notch1 and Hes1 related to Notch signaling pathway in the hippocampus,and synaptic plasticity-related proteins synaptophysin(SYN),postsynaptic density protein-95(PSD-95)and brain-derived neurotrophic factor(BDNF)expression.RESULTS AND CONCLUSION:Electroacupuncture intervention significantly improved learning and memory impairment in mice with radiation-induced brain injury.The positive expression of BrdU in the model group was significantly lower than that in the blank group(P<0.01),and the positive expression of BrdU in the electroacupuncture group was significantly increased(P<0.01).The expression levels of Notch1,Hes1,PSD-95,SYN and BDNF were lower in the model group than that of the blank group(P<0.01,P<0.01,P<0.01,P<0.05,P<0.01).Compared with the model group,the expression levels of Notch1,PSD-95,SYN and BDNF increased in the electroacupuncture group(P<0.01,P<0.01,P

关 键 词：电针 放射性脑损伤 突触可塑性 NOTCH 信号通路 C57BL/6J小鼠 学习 记忆 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]