组织型转谷氨酰胺酶在大鼠心肌梗死后心肌纤维化组织中的表达及意义  

Expression and significance of tissue-type transglutaminase in myocardial fibrosis in rats after myocardial infarction

作　　者：孙润锋 陈云 谢印军 李敏[2] Sun Runfeng;Chen Yun;Xie Yinjun;Li Min(Department of Cardiology,People's Hospital of Lianyungang City,Jiangsu Province,Lianyungang 222300,China;不详)

机构地区：[1]连云港市人民医院心内科,连云港222300 [2]连云港市第二人民医院,连云港222023

出　　处：《中国循证心血管医学杂志》2020年第10期1240-1243,共4页Chinese Journal of Evidence-Based Cardiovascular Medicine

摘　　要：目的探讨组织型转谷氨酰胺酶(tTG)在大鼠心肌梗死后心肌纤维中的表达,分析其在心肌梗死后心肌纤维化中的作用与意义。方法选取30只8周龄雄性SD大鼠,随机均分为心肌梗死组和假手术组2组,其中心肌梗死组采用冠状动脉(冠脉)左前降支结扎方法构建,假手术组只缝针不结扎冠脉。造模4周后超声心动图检测心功能,检测tTG与纤维蛋白1在RNA水平中的表达,tTG及纤维蛋白1在蛋白水平的表达。结果与假手术组相比,心肌梗死组大鼠心室明显扩张[左心室收缩末期内径(LVESD):(4.88±0.36)mm vs.(1.79±0.28)mm,左心室舒张末期内径(LVEDD)为(6.36±0.42)mm vs.(3.25±0.32)mm],差异均有统计学意义(P<0.05);心功能严重受损[左心室射血分数(EF):(80.75±5.76)%vs.(28.64±3.14)%,短轴缩短率(FS):(42.23±4.13)%vs.(21.51±2.32)%],差异均有统计学意义(P<0.05);心肌组织纤维化程度加重,心肌胶原容积分数增加[(2.4±0.25)%vs.(11.7±0.32)%],存在统计学差异(P<0.05);与正常大鼠相比,患有心肌梗死的大鼠心肌组织中tTG的转录水平和蛋白水平显著增加,同时纤维蛋白1的表达在转录水平和蛋白水平显著增加,存在统计学差异(P<0.05)。结论转谷氨酰胺酶在梗死后心肌纤维化过程中发挥了重要作用,其与纤维蛋白1共同参与心肌纤维化的过程;转谷氨酰胺酶可能成为心肌纤维化诊断和治疗靶点。Objective To investigate the expression of tissue-type transglutaminase(tTG)in myocardial fibers in rats after myocardial infarction(MI),and analyze the effect and significance of tTG in myocardial fibrosis.Methods SD rats(n=30,aged 8 weeks old)were selected and randomly divided into MI group and sham-operation group.The MI group was given ligation of coronary left anterior descending branch for modeling,and shamoperation group was just given stitch without ligation.After modeling for 4 weeks,heart function was examined by using echocardiogram,and the mRNA and protein expressions of tTG and fibrin 1 were detected.Results Compared with sham-operation group,there was significant ventricular dilation[LVESD:(4.88±0.36)mm vs.(1.79±0.28)mm,LVEDD:(6.36±0.42)mm vs.(3.25±0.32)mm]in MI group(P<0.05),severe damage of heart function[LVEF:(80.75±5.76)%vs.(28.64±3.14)%,LVFS:(42.23±4.13)%vs.(21.51±2.32)%in MI group(P<0.05)],and exacerbation of myocardial fibrosis and increase of myocardial collagen volume fraction[(2.4±0.25)%vs.(11.7±0.32)%]in MI group(P<0.05).The mRNA and protein expressions of tTG and fibrin 1 increased significantly in MI group compared with sham-operation group(P<0.05).Conclusion The effect of tTG is important in the process of myocardial fibrosis after MI,and tTG and fibrin 1 take part in myocardial fibrosis together.Tissue-type transglutaminase may be a diagnostic and therapeutic target of myocardial fibrosis.

关 键 词：组织型转谷氨酰胺酶 纤维蛋白1 心肌纤维化 心肌梗死 大鼠 

分 类 号：R542.22[医药卫生—心血管疾病]