利拉鲁肽对MC3T3-E1前成骨细胞增生分化过程中内质网应激的影响  

Effects of liraglutide on endoplasmic reticulum stress during the proliferation and differentiation of MC3T3-E1 cells

作　　者：杨明明 解合兰[2] 朱慧静[2] 王惠丽[2] 朱昕 程霖[2] 杨乃龙[1] YANG Ming-ming;XIE He-lan;ZHU Hui-jing;WANG Hui-li;ZHU Xin;CHENG Lin;YANG Nai-long(Department of Endocrinology,the Affiliated Hospital of Qingdao University,Qingdao 266000,Shandong,China;Department of Endocrinology,Heze Municiple Hospital,Heze 274031,Shandong,China)

机构地区：[1]青岛大学附属医院内分泌科,山东青岛266000 [2]菏泽市立医院内分泌科,山东菏泽274031

出　　处：《中华骨质疏松和骨矿盐疾病杂志》2020年第5期440-445,共6页Chinese Journal Of Osteoporosis And Bone Mineral Research

摘　　要：目的探讨利拉鲁肽(liraglutide)对MC3T3-E1前成骨细胞增生分化的影响及其机制。方法体外培养MC3T3-E1前成骨细胞,给予不同浓度的利拉鲁肽(0、10-6、10-7、10-8、10-9 mol/L),24 h后用CCK8法检测细胞的增生活性,48 h后用碱性磷酸酶试剂盒测定碱性磷酸酶(alkaline phosphatase,ALP)活力;给予最适浓度10-7 mol/L利拉鲁肽处理MC3T3-E1细胞0、1、3、5、10 h,实时定量PCR技术检测BIP、XBP1基因的表达水平。结果与对照组相比,10-6、10-7、10-8、10-9mol/L利拉鲁肽组细胞增生活性A值分别升高了0.042、0.073、0.038、0.032(P<0.05),10-7 mol/L利拉鲁肽组A值升高最显著;与对照组相比,10-6、10-7、10-8、10-9 mol/L利拉鲁肽组ALP活性分别升高了0.400、0.717、0.321、0.303(P<0.05),10-7 mol/L利拉鲁肽组ALP活性升高最显著。与0 h比较,10-7 mol/L利拉鲁肽处理后,BIP基因的表达随时间的增加而增高,1和3 h无显著变化(P>0.05),5 h(1.983±0.230,P<0.01)和10 h(2.469±0.256,P<0.01)表达明显增加;XBP1基因表达在1 h无显著变化(P>0.05),3 h显著升高(3.449±0.246,P<0.01),5 h有下降趋势(1.597±0.259,P<0.01),10 h又升高(2.412±0.274,P<0.01)。结论利拉鲁肽可能通过上调BIP、XBP1基因的表达,促进MC3T3-E1细胞的增生与分化。Objective To explore the roles and mechanism of liraglutide on the proliferation and differentiation of the MC3T3-E1 cells.Methods MC3T3-E1 cells were cultured in vitro with different concentrations of liraglutide(0,10-6,10-7,10-8,10-9 mol/L),after 24 h cell proliferation was detected with cell counting kit-8(CCK8),alkaline phosphatase(ALP)activity was determined using the ALP kit after 48 h.Cultures were treated for 0,1,3,5,and 10 hours with the optimal concentration of liraglutide(10-7 mol/L),and the expression of BIP and XBP1 mRNA were measured by the qRT-PCR.ResultsCompared with the control group,the A values of 10-6,10-7,10-8,10-9 mol/L liraglutide groups increased respectively by 0.042,0.073,0.038,0.032(P<0.05).The A values of 10-7mol/L liraglutide group increased most significantly.Compared with the control group,the ALP activity of 10-6,10-7,10-8,10-9mol/L liraglutide groups increased respectively by 0.400,0.717,0.321,0.303(P<0.05).Liraglutide(10-7 mol/L)had the strongest effects.After liraglutide(10-7 mol/L)treatment,compared to unstimulated cells(time 0),the expression of BIP mRNA increased with time.BIP mRNA levels were not altered at 1 h and 3 h(P>0.05),increased significantly at 5 h(1.983±0.230,P<0.01)and 10 h(2.469±0.256,P<0.01).The expression of XBP1 mRNA was not altered at 1 h(P>0.05),and increased significantly at 3 h(3.449±0.246,P<0.01),decreasing at 5 h(1.597±0.259,P<0.01),increased at 10 h again(2.412±0.274,P<0.01).Conclusion Liraglutide could promote the proliferation and differentiation of MC3T3-E1 cells through up-regulating the mRNA expression of BIP and XBP1.

关 键 词：成骨细胞 利拉鲁肽 内质网应激 骨质疏松 糖尿病 

分 类 号：R681[医药卫生—骨科学]