车前子对腹泻模型大鼠小肠组织水通道蛋白4表达的影响  

Effect of Plantaginis semen on the expressions of AQP4 gene and protein in small intestine of diarrhea rats

作　　者：彪雅宁 王瑞龙 储心乔 胡巍巍[2] 张纳博 韩雪 张一昕 刘晨 Biao Yaning;Wang Ruilong;Chu Xinqiao;Hu Weiwei;Zhang Nabo;Han Xue;Zhang Yixin;Liu Chen(School of Pharmacy,Hebei University of Chinese Medicine,Application Technology Research Center of Traditional Chinese Medicine Prescription in University of Hebei Province,Shijiazhuang 050200,China;Department of Clinical Medicine,Shijiazhuang College of Medical,Shijiazhuang 050599,China)

机构地区：[1]河北中医学院药学院,河北省高校中药组方制剂应用技术研究中心,石家庄050200 [2]石家庄医学高等专科学校临床医学院,050599

出　　处：《国际中医中药杂志》2020年第8期758-762,共5页International Journal of Traditional Chinese Medicine

基　　金：河北省中医药管理局科研计划项目(2018210);中央财政公共卫生专项“2018年中药资源普查项目”(Z135080000022);国家级大学生创新创业训练计划项目(201714432001)。

摘　　要：目的观察车前子对腹泻模型大鼠小肠组织水通道蛋白4(aquaporin4,AQP4)基因及蛋白表达的影响,探讨其作用机制。方法将60只大鼠按体重随机分为正常组、模型组、氢氯噻嗪组和车前子低、中、高剂量组。除正常组外,其余各组大鼠采用番泻叶灌胃法复制腹泻模型。车前子低、中、高剂量组灌胃0.95、1.90、3.80 g/kg车前子配方颗粒溶液,氢氯噻嗪组大鼠灌胃氢氯噻嗪混悬液9 mg/kg,按1 ml/100 g大鼠体重灌胃,连续灌胃14 d。实验结束后,记录各组大鼠粪便性状和大便次数,计算稀便率、平均稀便级和腹泻指数;采集小肠组织,采用HE染色法观察小肠组织病理形态学改变,采用PCR法和Western blot法检测小肠组织中AQP4基因和蛋白表达。结果与模型组比较,车前子低、中、高剂量组大鼠稀便率[(50.89±6.17)%、(41.14±4.48)%、(36.37±4.81)%比(67.45±7.35)%]、平均稀便级[(2.16±0.34)级、(1.73±0.28)级、(1.52±0.25)级比(2.63±0.29)级]、腹泻指数[(1.10±0.19)、(0.71±0.11)、(0.57±0.12)比(1.77±0.24)]降低(P<0.01);小肠黏膜损伤程度、充血和中性粒细胞浸润现象均减轻;小肠组织AQP4 mRNA[(0.48±0.10)、0.69±0.12)、(0.97±0.15)比(0.21±0.03)]、AQP4蛋白[(0.59±0.08)、(0.64±0.09)、(0.78±0.11)比(0.32±0.05)]表达升高(P<0.01)。结论车前子可上调AQP4 mRNA和蛋白表达水平、增加小肠对水的吸收、调节水液代谢,从而发挥止泻作用。Objective To explore the effect of Plantaginis semen on the expressions of AQP4 gene and protein in small intestine of diarrhea rats.Methods Sixty Sprague-Dawley male rats were randomly divided into normal control group,model control group,hydrochlorothiazide group,and low-,medium-,and high-dose Plantaginis semen groups.Intragastric administration of Senna(20 ml/kg)was implemented in the morning for 5 groups except the normal group.The rats in the low-,medium-,and high-dose Plantaginis semen groups were intragastrically administered with 0.95,1.9,3.8 g/kg Plantaginis semen formula granule solution,while the rats in hydrochlorothiazide group were given hydrochlorothiazide suspension 9 mg/kg by gavage according to 1 ml/100 g body weight for 14 days.The loose stool rate,average ranking of loose stool,and diarrhea index were compared according to the fecal traits and stool times after 14 days of treatment,and the small intesine tissue were collected.Hematoxylin-eosin(HE)staining to observe the pathological morphological changes in small intestine,and Quantiative Real-time PCR and Western blot were applied to detect the gene and protein expressions of AQP4.Results Compared with the model group,the loose stool rate(50.89%±6.17%,41.14%±4.48%,36.37%±4.81%vs.67.45%±7.35%),the average ranking of loose stool(2.16±0.34,1.73±0.28,1.52±0.25 vs.2.63±0.29),and the diarrhea index(1.10±0.19,0.71±0.11,0.57±0.12 vs.1.77±0.24)of rats in each group of Plantaginis semen significantly decreased(P<0.05);the degree of intestinal mucosal injury,hyperemia and neutrophil infiltration were alleviated;the expressions of AQP4 mRNA(0.48±0.10,0.69±0.12,0.97±0.15 vs.0.21±0.03),and the protein of AQP4(0.59±0.08,0.64±0.09,0.78±0.11 vs.0.32±0.05)in the small intestine tissue of Plantaginis semen groups significantly increased(P<0.05).Conclusions Plantaginis semen has antidiarrheal effect,and its mechanism is related to up-regulation the gene and protein expressions of AQP4,addition of water absorption and promotion of water and fl

关 键 词：腹泻 车前子 水通道蛋白4 大鼠 

分 类 号：R285.5[医药卫生—中药学]