HBsAg阳性孕产妇miR-122 rs7227488基因多态性与HBV宫内感染发生的相关性  

Correlation between miR-122 rs7227488 gene polymorphism and HBV intrauterine infection in HBsAg-positive pregnant women

作　　者：林婧[1] 夏霖[1] 冯地路[1] LIN Jing;XIA Lin;FENG Di-lu(Department of Obstetrics and Gynecology,Xiyuan Hospital of Union Hospital affiliated to Huazhong University of science and technology,Wuhan 430000,China)

机构地区：[1]华中科技大学附属协和医院西院妇产科,武汉430000

出　　处：《肝脏》2020年第10期1034-1037,共4页Chinese Hepatology

基　　金：国家自然科学基金青年科学基金(81601260)。

摘　　要：目的探讨HBsAg阳性孕产妇miR-122启动子基因多态性与乙型肝炎病毒(HBV)宫内感染易感性的关系。方法选取2017年10月至2019年7月华中科技大学附属协和医院西院妇产科行产前检查并分娩的141对HBsAg阳性孕妇及新生儿为研究对象,收集孕妇一般资料(年龄、分娩时孕周、分娩方式、新生儿性别等),将HBsAg阳性孕妇分为HBV宫内感染组47例,无HBV宫内感染组94例,运用聚合酶链反应-限制性片段长度多态性(PCR-RELP)检测研究对象miR-122启动子rs7227488基因多态性,分析miR-122启动子rs7227488基因多态性与新生儿HBV感染的相关性,采用logistic回归分析影响HBsAg阳性孕产妇发生HBV宫内感染发生的危险因素。结果Hardy-Weinberg平衡定律显示,无HBV宫内感染组与HBV宫内感染组G/A各基因型符合Hardy-Weinberg平衡定律(均P>0.05);与无HBV宫内感染组44.6%相比,HBV宫内感染组55.3%rs7227488位点G/A点突变率显著升高(P<0.05);二元logistic回归分析显示,HBV DNA含量、miR-122基因多态性位点rs7227488 AA型是HBsAg阳性孕产妇的HBV宫内感染的危险因素(OR:1.512、1.898,均P<0.05)。结论HBsAg阳性孕产妇miR-122 rs7227488 AA基因型与HBV宫内感染的发生密切相关。Objective To investigate the relationship between rs7227488 polymorphism site at the gene promoter region of miR-122 and the susceptibility of intrauterine infection in HBsAg-positive pregnant women.Methods One hundred and fourty-one HBsAg positive pregnant women who underwent prenatal examination and deliveried were selected in this study.General data of the pregnant women(age,gestational week at delivery,mode of delivery,sex of newborn)were collected.The HBsAg-positive pregnant women were divided into HBV intrauterine infection group(47 cases)and non-HBV intrauterine infection group(94 cases)according to the occurance of HBV infection in their neonates.The rs 7227488 polymorphism of miR 122 gene in all subjects was detected using polymerase chain reaction-restriction fragment length polymorphism(PCR-RELP).Risk factors of HBV intrauterine infection in HBsAg positive pregnant women and the correlation between rs7227488 and neonatal HBV infection were analyzed by logistic regression analysis.Results The G/A genotypes of rs7227488 in non-HBV intrauterine infection group and HBV intrauterine infection group were accordant with Hardy-Weinberg equilibrium law(P>0.05).The G/A mutation rate of rs7227488 in non-HBV intrauterine infection group(44.6%)was significantly higher than that of the HBV intrauterine infected group(55.3%)(P<0.05).Bivariate logistic regression analysis showed that HBV DNA load and rs7227488 AA type were risk factors for intrauterine HBV infection in HBsAg-positive pregnant women(Or=1.512,1.898,all P<0.05).Conclusion The miR-122 rs7227488 AA genotype of HBsAg-positive pregnant and lying-in women are risk factors of intrauterine HBV infection.

关 键 词：乙型肝炎病毒 微小RNA-122 基因多态性 宫内感染 

分 类 号：R71[医药卫生—妇产科学]