西洋参蒸制前后人参皂苷类成分变化及活性比较研究  

Comparative study on changes of ginsenosides and activities of American ginseng before and after steaming

作　　者：安琪 郭梅 申亚君 张莹 王瑞鸾 郭龙 郑玉光 张丹[1,2] AN Qi;GUO Mei;SHEN Ya-jun;ZHANG Ying;WANG Rui-luan;GUO Long;ZHENG Yu-guang;ZHANG Dan(College of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Hebei Chemical and Pharmaceutical College,Shijiazhuang 050026,China)

机构地区：[1]河北中医学院药学院,河北石家庄050200 [2]河北中医学院河北省中药炮制技术创新中心,河北石家庄050200 [3]河北化工医药职业技术学院,河北石家庄050026

出　　处：《中国中药杂志》2020年第18期4404-4410,共7页China Journal of Chinese Materia Medica

基　　金：中央本级重大增减支项目(2060302);中央财政公共卫生专项“中药资源普查项目”(Z135080000022);河北省自然科学基金青年项目(H2018423029);河北省中医药管理局科研计划项目(2015079)。

摘　　要：采用高效液相色谱法(HPLC),结合中药色谱指纹图谱相似度评价系统建立西洋参药材的化学指纹图谱,并采用化学计量法(聚类分析、主成分分析、正交偏最小二乘判别分析等)对所得结果进行分析;同时,进行了初步的抗氧化和抗结直肠癌细胞增殖活性的研究。通过比对炮制前后西洋参药材的指纹图谱表明,该文定量的8个人参皂苷类成分中5个成分的含量升高,分别为人参皂苷Rc,Rg2,Rb2,Rb3,Rd,蒸制后还新产生了一个成分。活性研究表明蒸西洋参抗氧化活性和抗结肠癌细胞增殖活性均优于生西洋参。研究结果表明,该实验所采用的西洋参的蒸制方法具有较好的稳定性与重复性,且西洋参蒸制后产生了与人参加工为红参后相似的变化,为扩大西洋参的应用范围提供了一定的参考。The chemical fingerprints of American ginseng were established by high performance liquid chromatography(HPLC)coupled with similarity evaluation system for chromatographic fingerprint of traditionanl Chinese medicine.The results were analyzed with use of stoichiometry methods(cluster analysis,principal component analysis,and orthogonal partial least squares discriminant analysis),and meanwhile,a preliminary study on the antioxidant and anti-proliferation activity of colorectal cancer cells was conducted.By comparing the fingerprints of American ginseng before and after processing,the contents of five components in the eight ginseno-sides quantified in this paper increased,including ginsenoside Rc,Rg2,Rb2,Rb3 and Rd,respectively,and a new component was produced after steaming.The activity study showed that steamed American ginseng had better antioxidant activity and anti-proliferation activity of colorectal cancer cells than raw American ginseng.The research results show that the steaming method of American ginseng used in this experiment has good stability and reproducibility,and the steaming of American ginseng produces similar changes as artificial red ginseng,which provides a certain reference for expanding the application range of American ginseng.

关 键 词：蒸制西洋参 人参皂苷 HPLC指纹图谱 化学成分 活性 

分 类 号：R284.1[医药卫生—中药学]