原肌球蛋白2表达变化对主动脉夹层影响的实验研究  

Expression of tropomyosin 2 in aortic dissection tissue

作　　者：钟晓璇 魏翔[1] 蒋丁胜 朱学海[1] 刘立刚[1] Zhong Xiaoxuan;Wei Xiang;Jiang Dingsheng;Zhu Xuehai;Liu Ligang(Divison of Cardiothoracic and Vascular Surgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院心脏大血管外科,武汉430300

出　　处：《中华心血管病杂志》2020年第9期777-781,共5页Chinese Journal of Cardiology

基　　金：国家自然科学基金(81670050)。

摘　　要：目的探究原肌球蛋白2(TPM2)在主动脉夹层患者中的表达情况及其临床意义。方法入选自2015年起于华中科技大学同济医学院附属同济医院住院治疗、经胸腹主动脉CT血管成像明确诊断为Stanford A型主动脉夹层的患者13例,即夹层组,在手术中剪取患者的主动脉壁组织。选取心脏移植患者10例作为对照组,取其正常主动脉壁组织。采用苏木素-伊红(HE)染色和弹性纤维EVG染色观察两组的主动脉壁结构和弹力纤维及胶原纤维情况,实时荧光定量PCR检测两组标本中TPM2基因的mRNA相对表达量;采用Western blot及免疫组织化学染色法检测两组样本中TPM2蛋白表达水平和亚细胞定位,采用image J软件采集图片上深染的各个点的积分光密度值(IOD),计算平均密度(%,IOD/目标分布区域的面积)。结果HE染色及EVG染色显示,与对照组比较,夹层组患者的主动脉壁中层退变,弹力纤维断裂、胶原纤维沉积。PCR结果显示夹层组患者主动脉壁组织中TPM2基因的mRNA相对表达量高于对照组(P<0.05)。Western blot及免疫组织化学染色结果均显示夹层组患者主动脉壁组织中TPM2的蛋白表达水平高于对照组[平均密度为(9.73±1.20)%比(0.11±0.04)%,P<0.05],且TPM2主要表达在细胞质中。结论主动脉夹层患者主动脉壁中TPM2的mRNA和蛋白表达水平偏高,提示TPM2可能参与主动脉夹层的发生发展。Objective To investigate the expression pattern of tropomyosin 2(TPM2)in aorta of patients with aortic dissection and explore its clinical implication.Methods Thirteen cases with acute type A aortic dissection(TAAD)diagnosed by transabdominal aortic angiography from 2015 in Tongji Hospital were included.During the operation,the aortic wall tissues of these patients were collected.Ten patients with heart transplantation were selected as control group,and normal aortic wall tissues were taken.The hematoxylin-eosin(HE)and Verhoeff's Van Gieson(EVG)staining were performed to observe the morphological changes of aorta.The mRNA expression level of TPM2 was measured by real-time fluorescent quantitative-PCR,and the protein levels of TPM2 were detected by Western blot and immunohistochemical staining.lmage The J software was used to collect the optical density values of each point on the image,obtain the integrated optical density(I0D)value,and calculate the average density(%,I0D/area of the target distribution area).Results HE and EVG staining revealed medial degeneration and broken elastic fiber in aorta of TAAD patients.The mRNA expression levels of TPM2 were significantly upregulated in aorta of TAAD patients as compared to the control group(P<0.05),so as the TPM2 protein expression levels((9.73+1.20)%vs.(0.11+0.04)%,P<0.05).And TPM2 was mainly expressed in cytoplasm.Conclusion The increased expression of TPM2 in TAAD patients hints that TPM2 might be involved in the pathogenesis of aortic dissection.

关 键 词：主动脉夹层 原肌球蛋白2 主动脉平滑肌细胞 

分 类 号：R543.1[医药卫生—心血管疾病]