鳜·斑鳜及杂交鳜(鳜♀×斑鳜♂)的染色体组型研究  

Study on the Karyotypes of Siniperca chuatsi,Siniperca scherzeri and Their Hybrids[S.chuatsi(♀)×S.scherzeri(♂)]

作　　者：吕青兰 薛源 史杨白[2] 刘苗 金锐铭 丁淑燕[2] 陆仲逸 黄鹤忠[1] LÜ Qing-lan;XUE Yuan;SHI Yang-bai(School of Biology&Basic Medical Sciences,Soochow University,Suzhou,Jiangsu 215123;Jiangsu Freshwater Fisheries Research Institute,Nanjing,Jiangsu 210000)

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123 [2]江苏省淡水水产研究所,江苏南京210000 [3]常州市技丰水产良种繁育场,江苏常州213115

出　　处：《安徽农业科学》2020年第21期95-99,共5页Journal of Anhui Agricultural Sciences

基　　金：江苏省重点研发计划(现代农业)项目(BE2017311,BE2018398);苏州大学校级大学生创新创业训练计划项目(2019xj069);江苏省渔业科技项目(cx(18)2012-01)。

摘　　要：鳜鱼(Siniperca chuatsi)、斑鳜(Siniperca scherzeri)及其杂交鳜(鳜♀×斑鳜♂)为研究对象,采用植物血球凝集素(PHA)和秋水仙素体内注射获得大量有丝分裂中期相的头肾细胞,直接制片法制备染色体标本后进行染色体组型的研究和分析。结果表明:3种鳜鱼染色体数目皆为2n=48,均未观察到随体、次缢痕或其他特殊标志的染色体。鳜、斑鳜和杂交鳜的核型公式分别为2n=8sm+10st+30t(NF=56)、2n=2m+8sm+10st+28t(NF=58)和2n=2sm+12st+34t(NF=50)。该研究鳜和斑鳜的染色体组型与其他水系种群相比有所差异,呈现多态性。首次报道的杂交鳜染色体组型与父母本皆有不同,显示了杂交后代复杂的染色体变异。Taking Siniperca chuatsi,Siniperca scherzeri and their hybrids[S.chuatsi(♀)×S.scherzeri(♂)]as the research objects,a large number of head kidney cells in mitotic metaphase were obtained by PHA and colchicine injection in vivo,and then the chromosome samples were prepared by direct production method,the karyotypes were studied and analyzed.The results showed that the chromosome number of three species of mandarin fish were all 2n=48,and no satellite,secondary constriction or other special markers were observed.The karyotype formulae of S.chuatsi,S.scherzeri and their hybrids were 2n=8sm+10st+30t(NF=56),2n=2m+8sm+10st+28t(NF=58)and 2n=2sm+12st+34t(NF=50),respectively.In this experiment,the karyotypes of S.chuatsi and S.scherzeri were different from those of other river populations,showing polymorphism.The karyotype of hybrid mandarin fish reported for the first time was different from that of its parents,which showed the chromosomal intricately mutated in hybrid offspring.

关 键 词：鳜 斑鳜 杂交鳜 染色体组型 

分 类 号：S917.4[农业科学—水产科学]