翘嘴鳜·斑鳜·杂交鳜不同组织3种同工酶特征研究  

Study on Isozymes Characteristics of Different Tissues of Siniperca chuatsi,Siniperca scherzeri and Their Hybrid Mandarin Fish

作　　者：梁家僖 薛源 史杨白[2] 金锐铭 王莹莹 陆仲逸 丁淑燕[2] 黄鹤忠[1] LIANG Jia-xi;XUE Yuan;SHI Yang-bai(School of Biology&Basic Medical Sciences,Soochow University,Suzhou,Jiangsu 215123;Jiangsu Province Freshwater Fisheries Research Institute,Nanjing,Jiangsu 210000)

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123 [2]江苏省淡水水产研究所,江苏南京210000

出　　处：《安徽农业科学》2020年第21期109-114,共6页Journal of Anhui Agricultural Sciences

基　　金：苏州大学校级大学生创新创业训练计划(2019xj069);江苏省重点研发计划(现代农业)(BE2017311);江苏省渔业科技类项目(Y2017-38);江苏省重点研发计划(现代农业)(BE2018398)。

摘　　要：揭示翘嘴鳜、斑鳜和杂交鳜F 1[翘嘴鳜(♀)×斑鳜(♂)]的同工酶生化遗传特性。采用非变性聚丙烯酰胺凝胶电泳(Native-PAGE)检测技术,分别进行了斑鳜、翘嘴鳜3个组织(肌肉、肝脏、肾脏)以及杂交鳜F 1代5个组织(肌肉、肝脏、心脏、肾脏、脑)的3种同工酶(LDH 1.1.1.27、MDH1.1.1.37、EST3.1.1.1)酶谱特征的研究。3种同工酶均存在较稳定的物种和组织特异性表达的特征;杂交鳜F 1代同工酶酶谱的组织表达与双亲(斑鳜、翘嘴鳜)之间存在明显的亲缘关系和特异性,除了斑鳜和翘嘴鳜肾脏中的MDH同工酶谱以外,3个不同鳜种间的酶谱特征均存在较明显的种属特异性,肝脏、肾脏中的LDH和EST同工酶谱以及肌肉中的EST同工酶谱均存在显著且稳定种属特异性差异表达,可作为鉴别3种鳜鱼种质特性的重要遗传生化参考指标。该研究结果为鳜鱼的种质鉴定和杂交育种提供了一定的理论依据,并具有较好的应用价值。In order to reveal the isozyme genetic characteristics of Siniperca chuatsi,Siniperca scherzeri and hybrid mandarin fish[Siniperca chuatsi(♀)×Siniperca scherzeri(♂)],using native polyacrylamide gel vertical electrophoresis technology,three isozymes(LDH 1.1.1.27,MDH1.1.1.37,EST3.1.1.1)of five tissues(muscle,liver,heart,kidney,brain)of hybridization F 1 and three tissues(muscle,liver,kidney)of Siniperca chuatsi,Siniperca scherzeri were analyzed.The results showed that there were stable species and tissue specific expression characteristics in three isozymes.The tissue expression of isozyme of hybridization F 1 had obvious relationship and specificity with its parents[Siniperca chuatsi(♀)×Siniperca scherzeri(♂)].Except for the MDH isozyme zymogram of kidney of Siniperca scherzeri and Siniperca chuatsi,the isozyme characteristics of three species of mandarin fish showed significant species specificity.The LDH and EST isozyme zymogram of kidney and liver and EST isozyme zymogram of muscle had remarkable and stable species differential expression,which could be used as a key biochemical indicator to distinguish the germplasm characteristics of three species of mandarin fish.Therefore,the results of this study provided a theoretical basis for germplasm identification and hybrid breeding of these three species of mandarin fish,and had a good application value.

关 键 词：翘嘴鳜 斑鳜 杂交鳜 同工酶 

分 类 号：S917.4[农业科学—水产科学]