梯度洗脱波长切换HPLC同时测定茵栀解毒颗粒中3个成分的含量  

Simultaneous Determination of 3 Constituents in Yinzhi Jiedu Granules by Gradient Elution HPLC Method with Switching Wavelength

作　　者：郑举[1] 刘红云[2] 陈静[1] 洪功飞[1] ZHENG Ju;LIU Hong-yun;CHEN Jing(Anhui Province Animal Disease Prevention and Control Center,Hefei,Anhui 230091;Anhui Province Institute of Veterinary Drug and Feedstuff,Hefei,Anhui 230091)

机构地区：[1]安徽省动物疫病预防与控制中心,安徽合肥230091 [2]安徽省兽药饲料监察所,安徽合肥230091

出　　处：《安徽农业科学》2020年第21期198-201,共4页Journal of Anhui Agricultural Sciences

摘　　要：[目的]建立同时测定茵栀解毒颗粒中绿原酸、黄芩苷、栀子苷3个成分含量的方法。[方法]采用Agilent Eclipse XDB-C 18(4.6 mm×250 mm,5μm),以乙腈(A)~0.2%磷酸水溶液(B)为流动相,梯度洗脱,流速1 mL/min,柱温30℃,检测波长327 nm(0~9.5 min,测定绿原酸)、238 nm(9.5~14.0 min,测定栀子苷)、278 nm(14~30 min,测定黄芩苷)。[结果]各待测组分分离度良好;绿原酸、黄芩苷、栀子苷3个成分的进样量分别在5.01~100.00μg/mL(r=0.9997)、6.25~100.00μg/mL(r=0.9995)、10.86~400.00μg/mL(r=0.9993)与峰面积呈良好的线性关系;加样回收率分别为98.5%、100.0%、99.0%(n=6),RSD分别为0.2%、0.7%、0.4%。[结论]该试验建立的含量测定方法符合方法学验证要求,可用于茵栀解毒颗粒的3个指标性成分的同时测定。该方法简便、准确度高、重复性好,为进一步控制茵栀解毒颗粒的质量标准提供了依据。[Objective]To establish a method for the simultaneous determination of chlorogenic acid,baicalin and geniposide in Yinzhi Jiedu granules.[Method]The chromatographic separation was carried out on a Agilent Eclipse XDB-C 18 column(4.6 mm×250 mm,5μm)with a mobile phase consisting of acetonitrile(A)-0.2%phosphoric acid solution(B)in a gradient mode at a flow rate of 1 mL/min.Column temperature was 30℃,the UV detection wavelength was set at 327 nm for chlorogenic acid in the first 9.5 min,238 nm for geniposide during 9.5-14.0 min,278 nm for baicalin during 14-30 min.[Result]Excellent chromatographic separation was achieved and the ranges for linear correlation of chlorogenic acid,baicalin and geniposide were 5.01-100.00μg/mL(r=0.9997),6.25-100.00μg/mL(r=0.9995),10.86-400.00μg/mL(r=0.9993),respectively.The sample recovery rates were 98.5%,100.0%,and 99.0%(n=6),and the RSD were 0.2%,0.7%,and 0.4%,respectively.[Conclusion]This method is consistent with the method validation requirements,and can simultaneously determine the above 3 index components of Yinzhi Jiedu granules.The method is simple,accurate and repeatable,and provides a basis for further control of the quality standard of Yinzhi Jiedu granules.

关 键 词：茵栀解毒颗粒 绿原酸 黄芩苷 栀子苷 含量测定 高效液相色谱 梯度洗脱 波长切换 

分 类 号：R284.1[医药卫生—中药学]