基于1H-NMR代谢组学和抗糖尿病活性的小檗皮不同基原品种质量评价研究  

Quality evaluation of different Berberidis Cortex species based on ~1H-NMR metabolomics and anti-diabetic activity

作　　者：范刚[1] 李琪[2] 徐鑫梅 杜欢 徐僮 赖先荣[1] 杜蕾蕾[1] FAN Gang;LI Qi;XU Xin-mei;DU Huan;XU Tong;LAI Xian-rong;DU Lei-lei(College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学民族医药学院,四川成都611137 [2]成都中医药大学药学院,四川成都611137

出　　处：《中国中药杂志》2020年第19期4677-4685,共9页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81303310,81874370);国家重点研发计划项目(2019YFC1712302)。

摘　　要：为评价小檗皮4种主流基原品种的质量差异,该研究采用氢核磁共振(1H-NMR)代谢组学技术整体检测小檗皮药材中的初级和次级代谢产物,结合偏最小二乘法判别分析和方差分析筛选种间差异成分。此外,以高脂饲料和链脲佐菌素建立2型糖尿病大鼠动物模型,评价4个品种的抗糖尿病活性差异。结果从小檗皮药材中同时检测并鉴定了包括生物碱类、有机酸类、糖类及氨基酸类在内的16种化合物,并首次发现种间差异标志物(markers)为木兰花碱、药根碱、蟾毒色胺内盐和蔗糖。匙叶小檗和甘肃小檗具有较好的降血糖、改善胰岛素抵抗、提高胰岛素敏感性及抗炎作用,刺红珠有一定的抗糖尿病作用,而鲜黄小檗的抗糖尿病活性较差。从4个差异成分的含量及抗糖尿病活性评价结果可知,不同基原小檗皮药材的质量优劣为:匙叶小檗≈甘肃小檗>刺红珠>鲜黄小檗,该研究结果可为小檗皮药材的品种整理、质量标准建立及开发利用提供参考依据。今后应进一步深入研究匙叶小檗和甘肃小檗的抗糖尿病活性及其作用机制。To evaluate the quality differences of four mainstream species of Berberidis Cortex,~1H-NMR metabolomics was applied to detect its primary and secondary metabolites, and the partial least squares discriminant analysis and analysis of variance were integrated to screen differential metabolites between species. Furthermore, diabetic rat model was established by high fat diet and streptozotocin to assess differences in antidiabetic activities among the four species. Sixteen compounds were simultaneously detected and identified, including alkaloids, organic acids, carbohydrates and amino acids. Interspecific difference markers were revealed as magnoflorine, jateorhi-zine, bufotenidine and saccharose for the first time. Berberis vernae and B. kansuensis presented superior activities on reducing blood glucose level, improving insulin resistance, increasing insulin sensibility and anti-inflammation. B. dictyophylla showed moderate antidiabe-tic effect, while B. diaphana rendered inferior antidiabetic capacity. Based on the contents of four differential markers and the results of antidiabetic activity evaluation, the quality of four Berberidis Cortex species was ranked as B.vernae≈B.kansuensis>B. dictyophylla>B. diaphana. These results provided references for species collation, quality standard establishment and exploitation of Berberidis Cortex. The antidiabetic activities of B. vernae and B. kansuensis as well as their mechanisms of action merit further study in the future.

关 键 词：小檗皮 质量评价 1H-NMR代谢组学 2型糖尿病 多基原 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]