用于慢性伤口羧甲基壳聚糖/氧化葡甘聚糖/三七总皂苷复合海绵的制备及性能评价  

Preparation and performance evaluation of carboxymethyl chitosan/oxidized glucomannan/Panax notoginseng compound sponge dressing for chronic wound

作　　者：李洁 许楗桢 胡萍 雷琪琪 张文柠 敖宁建[1,2] Li Jie;Xu Jianzhen;Hu Ping;Lei Qiqi;Zhang Wenning;Ao Ningjian(College of Life Science and Technology,Jinan University,Guangzhou 510632,Guangdong Province,China;Key Laboratory of Biomaterials of Guangdong Education Department,Guangzhou 510632,Guangdong Province,China)

机构地区：[1]暨南大学生命科学技术学院,广东省广州市510632 [2]广东省教育厅生物材料重点实验室,广东省广州市510632

出　　处：《中国组织工程研究》2021年第16期2528-2534,共7页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技厅科技计划资助项目(2017A020211006),项目负责人:敖宁建;国家自然科学基金(20976068),项目负责人:敖宁建。

摘　　要：背景:前期研究显示,羧甲基壳聚糖/氧化葡甘聚糖复合海绵可作为载药性慢性伤口敷料的优良基材。目的:制备羧甲基壳聚糖/氧化葡甘聚糖/三七总皂苷复合海绵,评估其理化性能与生物相容性。方法:采用高碘酸钠氧化制备氧化葡甘聚糖;从三七超细粉中提取出三七总皂苷。先加入羧甲基壳聚糖与氧化葡甘聚糖作为混合原料,再分别加入占混合体系体积2%,6%,10%的三七总皂苷,采用冷冻干燥的方法制备羧甲基壳聚糖/氧化葡甘聚糖/三七总皂苷复合海绵,采用扫描电镜观察复合海绵的微观结构,检测复合海绵的孔隙率、蒸气透过率、三七总皂苷释放度、细胞相容性、抗菌性能与急性全身毒性反应。结果与结论:①扫描电镜显示,复合海绵内部有许多孔洞饱满、分布均匀的孔隙,三七总皂苷稳定均匀地附着在海绵的孔洞的内壁及连接处;②随着三七总皂苷比例的增加,复合海绵的吸水率、孔隙率与蒸气透过率逐渐增加;③3种复合海绵中的绝大部分三七总皂苷体外在13 h内可高效地释放出来;④体外培养3 d内,3种复合海绵上培养的成纤维细胞增殖率均在95%以上;⑤3种复合海绵对大肠杆菌与金黄色葡萄球菌均有抑制作用;3种复合海绵无急性全身毒性反应;⑥结果表明,羧甲基壳聚糖/氧化葡甘聚糖/三七总皂苷复合海绵有望成为优良的医用慢性伤口敷料。BACKGROUND:Previous studies have shown that carboxymethyl chitosan/oxidized glucomannan composite sponge can be used as an excellent substrate for drug-loaded chronic wound dressings.OBJECTIVE:To prepare carboxymethyl chitosan/oxidized glucomannan/Panax notoginseng composite sponge and evaluate its physical and chemical properties and biocompatibility.METHODS:Oxidized glucomannan was prepared by sodium periodate oxidation.Panax notoginseng saponins were extracted from Panax notoginseng powder.Carboxymethyl chitosan and oxidized glucomannan were first added as mixed raw materials,and then added separately to account for mixing system 2%,6%,10%of Panax notoginseng saponins.Freeze-drying method was used to prepare carboxymethyl chitosan/oxidized glucomannan/Panax notoginseng composite sponge.Scanning electron microscope was utilized to observe the microstructure of the composite sponge.Porosity,steam permeability,total saponin release rate of Panax notoginseng,cell compatibility,antibacterial properties and acute systemic toxicity were detected.RESULTS AND CONCLUSION:(1)Scanning electron microscope showed that there were many pores in the composite sponge that were full and evenly distributed.The total saponins of Panax notoginseng were stably and evenly attached to the inner walls and joints of the pores of the sponge.(2)As the proportion of total saponins of Panax notoginseng increased,the water absorption rate,porosity,and steam permeability gradually increased.(3)The vast majority of the total saponins of Panax notoginseng in the three kinds of composite sponges could be efficiently released in 13 hours in vitro.(4)Within 3 days after in vitro culture,the proliferation rate of fibroblasts was more than 95%.(5)Three kinds of compound sponges have an inhibitory effect on E.coli and Staphylococcus aureus,but do not have acute systemic toxicity.(6)The results show that carboxymethyl chitosan/oxidized glucomannan/Panax notoginseng composite sponge is expected to be an excellent medical chronic wound dressing.

关 键 词：材料 三七总皂苷 羧甲基壳聚糖 氧化葡甘聚糖 复合海绵 慢性伤口 生物安全性 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]