亲和肽在软骨组织工程中应用的新策略和问题  

New strategies and problems of affinity peptide in cartilage tissue engineering

作　　者：付力伟 杨振 李浩[1,2] 高仓健 眭翔 刘舒云[1] 郭全义[1] Fu Liwei;Yang Zhen;Li Hao;Gao Cangjian;Sui Xiang;Liu Shuyun;Guo Quanyi(Institute of Orthopedics,the First Medical Center,Chinese PLA General Hospital,Beijing Key Laboratory of Regenerative Medicine in Orthopedics,Key Laboratory of Musculoskeletal Trauma&War Injuries,PLA,Beijing 100853,China;Medical College of Nankai University,Tianjin 300071,China)

机构地区：[1]解放军总医院第一医学中心骨科研究所,骨科再生医学北京市重点实验室,全军骨科战创伤重点实验室,北京市100853 [2]南开大学医学院,天津市300071

出　　处：《中国组织工程研究》2021年第16期2569-2574,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家重点研发计划课题(2019YFA0110600),项目负责人:郭全义;国家自然科学基金(81772319),项目负责人:郭全义。

摘　　要：背景:随着软骨组织工程的发展,亲和肽由于其对某些软骨组织工程关键因素的特殊亲和作用受到了研究人员的关注。目的:综述各类亲和肽的筛选、鉴定及在软骨组织工程中的应用。方法:使用计算机检索中国知网全文数据库、万方全文数据库、PubMed中与亲和肽有关的文献,检索时限为2000年1月到2020年5月。以"Affinity peptide,cartilage tissue engineering,mesenchymal stem cell,scaffold"为英文检索词,"亲和肽,软骨组织工程,间充质干细胞,支架"为中文检索词,最终纳入66篇文献进行分析。结果与结论:许多特定氨基酸序列的多肽能够与一些细胞、因子、分子特异性地结合,具有亲和作用,根据作用对象不同可将其分为细胞亲和肽、因子亲和肽及细胞外基质分子亲和肽。在软骨组织工程中,亲和肽通过黏附细胞、募集因子和分子等作用增强组织工程的修复效果。在许多仿生软骨多层支架策略中,与特定分子相互作用的亲和肽对于模拟正常软骨环境有着重要作用,同时随着软骨组织工程技术的发展尤其是计算机辅助技术的应用,为亲和肽的使用提供了新的策略,但亲和肽在体内实验的停留时间、降解率、降解途径等还需要进一步的了解。BACKGROUND:With the development of cartilage tissue engineering,affinity peptides have attracted some attention because of their special affinity to some key factors of cartilage tissue engineering.OBJECTIVE:To review the screening and identification of various affinity peptides and their application in cartilage tissue engineering.METHODS:The articles related to affinity peptides in CNKI,Wanfang,and PubMed were searched by computer from January 2000 to May 2020."Affinity peptides,cartilage tissue engineering,mesenchymal stem cell,scaffold"in English and Chinese were used as key words.Finally,66 articles were included for analysis.RESULTS AND CONCLUSION:Many polypeptides with specific amino acid sequence can bind with some cells,factors and molecules,and have affinity.According to different targets,they can be divided into cell affinity peptide,factor affinity peptide and extracellular matrix molecular affinity peptide.Affinity peptides have been used in cartilage tissue engineering through screening and identification to enhance the repair effect of tissue engineering by adhering fine cells,recruitment factors and molecules.In many strategies of biomimetic cartilage multilayer scaffolds,affinity peptides that interact with specific molecules play an important role in simulating the environment of normal cartilage.At the same time,with the development of cartilage tissue engineering technology,especially the application of computer-aided technology,it provides a new strategy for the use of affinity peptides.However,the residence time,degradation rate and degradation pathway of affinity peptides in vivo are relatively few,which need to be further understood.

关 键 词：软骨 材料 亲和肽 软骨组织工程 支架 间充质干细胞 综述 

分 类 号：R459.9[医药卫生—治疗学] R318.08[医药卫生—临床医学]