湖泊浮游细菌与浮游植物的溶原病毒诱导率检测方法优化  

Optimization of detection method for estimation of lysogeny rate of bacterioplankton and phytoplankton

作　　者：聂先贝 彭开达 程斯婷 赵以军 廖明军 NIE Xian-bei;PENG Kai-da;CHENG Si-ting;ZHAO Yi-jun;LIAO Ming-jun(College of Civil and Environmental Engineering,Hubei University of Technology,Wuhan 430068,China;Hubei Key Laboratory of Ecological Restoration for River-Lakes and Algal Utilization,Wuhan 430068,China)

机构地区：[1]湖北工业大学土木建筑与环境学院,武汉430068 [2]河湖生态修复及藻类利用湖北省重点实验室,武汉430068

出　　处：《生态学杂志》2020年第10期3531-3536,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(51579092和31370148)资助。

摘　　要：溶原性感染作为浮游病毒生态学效应表达方式之一,常以24 h化学诱导得到的溶原性宿主可诱导率来量化。为了获得适合淡水环境的溶原性宿主可诱导率研究方法,本研究采集6个淡水湖泊水样,对每个水样采用直接法、滤膜法、滤膜振荡法和直接振荡法4种诱导培养方法,通过宿主诱导致死率计算6个淡水湖泊浮游细菌和浮游植物溶原诱导率。结果表明:无论是浮游细菌还是浮游植物,直接法获得的诱导结果普遍高于其他方法,表明游离病毒的裂解性感染能够显著影响溶原诱导率的测定结果;直接振荡法获得的6个湖泊溶原诱导率与滤膜法及滤膜振荡法获得结果间均无显著差异,说明实验中振荡培养能有效抑制裂解性病毒对宿主的裂解性感染。本研究通过直接诱导结合振荡培养能够获得与滤膜法一致的诱导效果,但相比于滤膜法,直接振荡法实验过程更加简单,是一种更为高效的测定方法。The frequency of lysogenic cell(FLC)is an important index representing viral ecological function.To explore a suitable method for freshwater FLC test,water samples from six freshwater lakes were collected and treated with different induction methods,including direct induction,induction combining filtration,induction combining filtration and oscillation,direct induction combining oscillation.The FLC was calculated from the induced host mortality.The results showed that the FLC under the direct induction method was significantly higher than those under other methods for both phytoplankton and bacterioplankton(P<0.05),indicating that the lytic infection under the direct induction had a significant effect on the FLC test.Thus,the direct induction method is not suitable for assessing freshwater samples.There was no significant difference in the results from other methods(P>0.05),suggesting that the oscillation method can be as efficient as filtration in inhibiting lytic infection.Furthermore,the oscillation method is more convenient than filtration.In conclusion,the direct induction combining oscillation method is a simple and efficient way to test the FLC in freshwater.

关 键 词：浮游植物 浮游细菌 浮游病毒 溶原诱导 

分 类 号：Q938.8[生物学—微生物学] Q948.8