不同发育时期大豆豆荚性状QTL动态分析  

Dynamic QTL analysis of pod traits in soybean at different developmental stages

作　　者：滕卫丽[1] 郭志文 郑立娜 付雪 王博[1] 董莹莹 张丹洋 刘赫禹 冯文婧 赵雪[1] 韩英鹏[1] 李文滨[1] TENG Weili;GUO Zhiwen;ZHENG Lina;FU Xue;WANG Bo;DONG Yingying;ZHANG Danyang;LIU Heyu;FENG Wenjing;ZHAO Xue;HAN Yingpeng;LI Wenbin(Institute of Soybean Research,Key Laboratory of Soybean Biology in Chinese of Ministry Education,Key Laboratory of Soybean Biology and Breeding(Genetics)of Chinese Agriculture and Rural Ministry,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学大豆研究所,大豆生物学教育部重点实验室,农业农村部东北大豆生物学与遗传育种重点实验室,哈尔滨150030

出　　处：《东北农业大学学报》2020年第10期1-9,共9页Journal of Northeast Agricultural University

基　　金：黑龙江省“百千万”工程科技重大专项(2019ZX16B01-1);国家自然科学基金项目(31471517);现代农业产业技术体系建设专项资金项目(CARS-04-PS04);国家十三五重点研发计划项目(2017YFD0101306-05)。

摘　　要：文章选用母本东农46和父本L-100构建重组自交群体127个家系为试验材料,利用全基因组重测序技术构建高密度遗传连锁图谱,结合群体豆荚长、宽、厚性状R3~R8时期表型数据,利用QTL IciMapping 4.1.0.0软件完备区间作图法分析非条件和条件QTL加性和上位性效应。结果表明,在豆荚3个性状发育过程中,共检测到40个非条件加性效应QTL,贡献率为4.35%~25.52%;检测到12个条件加性效应QTL,贡献率为3.80%~23.32%。同时检测到多个QTL位点在不同性状或不同生育时期表达,如QPL-7-1、QPL-12-2和QPL-20-1(QPW-20-1)等。通过上位性分析检测到豆荚长度143对、宽度185对和厚度34对QTL位点,贡献率最高分别达29.69%、24.73%和25.17%,表明多基因效应在豆荚生长发育过程中发挥重要作用。In this study,total 127 lines of recombinant inbred lines population from female parent Dongnong 46 and male parent L-100 were selected as materials,and a high-density genetic linkage map was constructed using the whole-genome resequencing technology.The inclusive complete interval mapping method of QTL IciMapping 4.1.0.0 software was used to analysis additive and epistatic effects of conditional and non-conditional QTL of pod length,pod width and pod thickness.The results showed that during the development of the three traits of the pod,a total of 40 non-conditional additive QTLs were detected with a contribution rate of 4.35%-25.52%,12 QTLs with a conditional additive effect were detected,with a contribution rate of 3.80%-23.32%.At the same time,many non-condition and condition QTLs were detected simultaneously in different traits or different periods,such as QPL-7-1,QPL-12-2 and QPL-20-1(QPW-20-1).Total 143 pairs QTL of length,185 pairs QTL of width and 34 pairs QTL of thickness were acquired by the epistasis analys,with the highest contribution rates reaching 29.69%,24.73%and 25.17%,respectively,which indicated that the polygenic effects played an important role in the growth and development of seed pods.

关 键 词：大豆 豆荚性状 QTL 加性效应 上位性效应 

分 类 号：S565.1[农业科学—作物学]