苹果属小金海棠WRKY48基因克隆与功能初步分析  

Clone and preliminary functional analysis of Malus xiaojinensis transcription factors gene WRKY48

作　　者：韩德果 周正一 杜漫 李铁梅 王爽 杨国慧 HAN Deguo;ZHOU Zhengyi;DU Man;LI Tiemei;WANG Shuang;YANG Guohui(School of Horticulture and Landscape,Northeast Agricultural University,Key Laboratory of Biology and Genetic Improvement Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,Harbin 150030,China)

机构地区：[1]东北农业大学园艺园林学院,农业农村部东北地区园艺作物生物学与种质创制重点实验室,哈尔滨150030

出　　处：《东北农业大学学报》2020年第10期36-44,共9页Journal of Northeast Agricultural University

基　　金：国家自然科学基金项目(31301757);黑龙江省自然科学基金联合引导项目(LH2019C031,LH2020C009);黑龙江省博士后启动基金项目(LBH-Q16020)。

摘　　要：试验从苹果属小金海棠(Malus xiaojinensis Cheng et Jiang)中克隆得到一条全长为1116 bp基因,命名为MxWRKY48。MxWRKY48蛋白包含371个氨基酸,亚细胞定位试验表明MxWRKY48是一个细胞核定位蛋白。实时荧光定量PCR结果表明,MxWRKY48在小金海棠新叶中表达量高于茎、根和成熟叶。在高铁(160μmol·L-1)、低铁(4μmol·L-1)、盐(200 mmol·L-1)、低温(2℃)处理时,MxWRKY48在苹果属小金海棠根和新叶表达量均显著上升。转基因试验结果表明,MxWRKY48基因过表达可显著提高转基因拟南芥对低铁、高铁耐受能力。在高铁和低铁胁迫处理时,转基因拟南芥根长、鲜重、铁元素和叶绿素含量显著高于野生型拟南芥。In this study,a gene with a total length of 1116 bp was isolated from Malus xiaojinensis Cheng et Jiang and named as MxWRKY48.MxWRKY48 encoded 371 amino acids.Subcellular localization revealed that MxWRKY48 was a nucleus localized protein.The qPCR result showed that the expression level of MxWRKY48 in new leaves was higher than that in stems,roots and mature leaves.The results also showed that expression level of MxWRKY48 increased obviously in new leaves and roots under high iron(160μmol·L-1),low iron(4μmol·L-1),salt(200 mmol·L-1)and low temperature(2℃)treatments.The results of transgenic study indicated that the overexpression of MxWRKY48 in transgenic A.thaliana could significantly improve its tolerance to low iron and/or high iron stress.When dealt with high Fe stress and/or low Fe stress,the overexpression of MxWRKY48 in transgenic A.thaliana also led to increased fresh weight and root length,as well as higher contents of Fe and chlorophyll.

关 键 词：小金海棠 MxWRKY48 高铁胁迫 低铁胁迫 

分 类 号：S661.1[农业科学—果树学] Q785[农业科学—园艺学]