四君子汤对人小肠上皮细胞辐射损伤的保护作用  

The protection effect of Sijunzi Decoction(Four Noble Drugs Decoction)on radiation damage of human intestinal epithelial cells

作　　者：王成芳 邵帅[1] 曲功霖[1] 齐雪松[1] 苟巧[1] WANG Chengfang;SHAO Shuai;QU Gonglin;QI Xuesong;GOU Qiao(National Institute for Radiological Protection,Chinese Centre for Disease Control and Prevention,Key Laboratory of Radiological Protection and Nuclear Emergency,China CDC,Beijing 100088)

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,辐射防护与核应急中国疾病预防控制中心重点实验室,北京100088

出　　处：《辐射防护》2020年第5期456-461,共6页Radiation Protection

摘　　要：放射性肠损伤,是腹盆腔恶性肿瘤放射治疗引起的常见并发症,严重影响患者的生活质量。已有研究显示,四君子汤对BALB/c小鼠的电离辐射损伤具有较好的保护作用,为进一步探讨其对肠道辐射损伤的影响,本文以人小肠上皮细胞HIEC-6为受试对象,研究四君子汤水提物对其辐射损伤的保护作用。实验结果显示,0.95 mg/mL的四君子汤水提物可显著提高受照HIEC-6细胞的存活率,并减少细胞凋亡,减缓细胞周期阻滞,其分子作用机制与调控Bcl-2和γH2AX的蛋白表达有关。此研究结果可为四君子汤治疗电离辐射引起的肠道损伤提供参考依据。Irradiation-induced intestinal injury is a common complication caused by radiotherapy of abdominal and pelvic malignant tumors,which seriously affects the quality of patients’life.It has been reported that Sijunzi Decoction(Four Noble Drugs Decoction)showed a protective effect on BALB/c mice after irradiation.To further explore the role of Sijunzi Decoction(Four Noble Drugs Decoction)in intestinal radiation damage,in this paper,human intestinal epithelial cells(HIEC-6)was used as the subject to investigate the protective effects of Sijunzi Decoction(Four Noble Drugs Decoction)on radiation damage.The experimental results showed that 0.95 mg/mL Sijunzi Decoction(Four Noble Drugs Decoction)could repair radiation damage on HIEC-6,reduce apoptosis,and slow down cell cycle block.The mechanism of Sijunzi Decoction(Four Noble Drugs Decoction)reducing radiation damage was related to the regulation on protein expression of Bcl-2 andγH2AX.This work provided a reference for the treatment of radiation-induced intestinal injury by using Sijunzi Decoction(Four Noble Drugs Decoction).

关 键 词：四君子汤 电离辐射 辐射防护 人肠上皮细胞 

分 类 号：R142.4[医药卫生—公共卫生与预防医学]