开心散提取物抗焦虑作用及其化学成分研究  

Anxiolytic Effect of Kaixin San Extract and Its Bioactive Components

作　　者：胡灵燕 张旗 蔡淑美 张烁 冯燕婷 陈玲凡 辛志明[1] HU Lingyan;ZHANG Qi;CAI Shumei;ZHANG Shuo;FENG Yanting;CHEN Lingfan;XIN Zhiming(Fujian Center for Safety Evaluation of New Drug,Fujian Medical University,Fuzhou 350122,China;School of Pharmacy,Fujian Medical University,Fuzhou 350122,China;Clinical Department,Fujian Medical University,Fuzhou 350122,China)

机构地区：[1]福建医科大学福建省新药安全性评价中心,福建福州350122 [2]福建医科大学药学院天然药物学系,福建福州350122 [3]福建医科大学临床医学部,福建福州350122

出　　处：《中医药信息》2020年第6期1-5,共5页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金青年项目(21705020);福建省卫生计生科研人才培养项目(2018-1-73);福建医科大学启航基金项目(2017XQ1015);福建医科大学高层次人才科研启动经费(XRCZX2017030)。

摘　　要：目的:探索开心散提取物对大鼠的抗焦虑作用以及药效物质基础。方法:将大鼠随机分为模型组(Con)、阳性组(地西泮)、开心散提取物高剂量组(12 g/kg)、中剂量组(6 g/kg)、低剂量组(3 g/kg),并通过足刺激(0.5 mA)建立大鼠焦虑模型,分别灌胃给药7 d。采用高架十字迷宫试验(EPM)和旷场实验(OFT)进行行为学实验,为进一步确证开心散的抗焦虑作用,以ELISA法检测大鼠5-羟色胺(5-HT)的含量。同时,通过UPLC-DAD-ESI-MS n初步推断其功效成分组的化合物。结果:行为学数据表明,在高架十字迷宫实验,与对照组相比,开心散给药组(12 g/kg,6 g/kg)的大鼠均表现得更加活跃好动(P<0.05);5-HT检测结果显示,与对照组相比,给药组显著提高大鼠脑内神经递质5-HT的含量,并具有统计学意义(P<0.05)。建立开心散提取物的UPLC色谱图以及UPLC-MS-MS n总离子流图,根据质谱裂解信息以及文献对比,共解析图谱得到39个化合物。结论:开心散提取物具有显著的抗焦虑作用,主要含远志寡糖酯、远志酮、远志皂苷、人参皂苷和茯苓三萜类等成分。Objective:To study the anti-anxiety effect of Kaixin San(KXS)extract in rats and to illuminate its potential pharmacodynamic material basis.Methods:Rats were randomly divided into the control group,Diazepam group and KXS extract groups of high-dose(12 g/kg),medium-dose(6 g/kg)and low-dose(3 g/kg).Anxiety model was established by claws stimulation in rats.After 7 days of administration,elevated plus maze(EPM)test and open field test(OFT)were used to examine potential anxiolytic effects.Hippocampal 5-hydroxytryptamine(5-HT)level was tested by ELISA.Moreover,the underlying constituents of KXS extract were identified by UPLC-DAD-ESI-MS n method.Results:The rats in the KXS extract groups(12 g/kg,6 g/kg)were more active in terms of OFT than those in the control group(P<0.05).In terms of the content of 5-HT,it was increased remarkably in the medication groups than that in the control group(P<0.05).In addition,39 potential constituents of KXS extract were identified by establishing chromatogram of UPLC and total ion current of UPLC-MS-MS n.Conclusion:KXS extract had obvious anxiolytic effects,which were mainly oligosaccharide esters,ketone and saponin of Yuanzhi,saponin of Renshen and triterpenes of Fuling.

关 键 词：开心散 5-HT 抗焦虑症 化学成分 LC-MS 

分 类 号：R284[医药卫生—中药学]