凉血通瘀方改善脑出血大鼠行为学评分、抗炎作用的研究  

Effect of Liangxue Tongyu Decoction on Behavior Scores and Anti-Inflammation in Rats with Intracerebral Hemorrhage

作　　者：黄天马 李建香[2] 顾恒[1] 过伟峰[1] HUANG Tianma;LI Jianxiang;GU Heng;GUO Weifeng(Nanjing University of Chinese Medicine,Nanjing 210046,China;Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine,Nanjing 210022,China)

机构地区：[1]南京中医药大学,江苏南京210046 [2]南京中医药大学附属南京市中医院,江苏南京210022

出　　处：《中医药信息》2020年第6期12-15,共4页Information on Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81704017);中央本级重大增减支项目(2060302);江苏省自然科学基金面上研究项目(BK20191410);南京市卫生科技发展专项资金项目(JQX18006);南京中医药大学“江苏高校护理学优势学科建设工程资助项目”(2019YSHL093)。

摘　　要：目的:观察凉血通瘀方对脑出血大鼠行为学评分的影响,并初步探索凉血通瘀方的抗炎作用机制。方法:选取SD大鼠60只,随机分为正常组、假手术组、模型组和凉血通瘀组,每组设定3个时间观察点,分别为用药24 h、72 h、120 h,每个时间点5只大鼠。观察使用凉血通瘀方干预前后,脑出血大鼠行为学评分的变化情况;采用ELISA法检测脑组织TNF-α、IL-1β的表达水平;免疫组织化学检测大鼠脑组织NF-κB的表达水平。结果:1)在72 h时间点,凉血通瘀组行为学评分已明显低于模型组(P<0.05),并持续降低。2)在用药24 h、72 h、120 h 3个时间点,凉血通瘀组的TNF-α、IL-1β均低于模型组(P<0.01)。3)在用药24 h、72 h、120 h,凉血通瘀组的NF-κB均低于模型组(P<0.01)。结论:凉血通瘀方能够调控脑出血大鼠NF-κB通路活化、抑制炎性因子的分泌,从而减轻炎症反应的发生、降低炎症的级联放大效应,改善了脑出血大鼠的行为学评分,发挥抗炎作用。Objective:To observe the effect of Liangxue Tongyu Formula on behavioral scores of rats with intracerebral hemorrhage and to explore the anti-inflammatory mechanism of Liangxue Tongyu Formula.Methods:60 SD rats were randomly divided into the normal group,the sham operation group,the model group and Liangxue Tongyu group.Three observation points were set in each group,which were 24 h,72 h and 120 h,with 5 rats at each time point.The changes of behavioral scores of rats with intracerebral hemorrhage were observed before and after the intervention of Liangxue Tongyu Decoction;the expressions of TNF-αand IL-1 in brain tissue were detected by ELISA;the expression of NF-κB in brain tissue was detected by immunohistochemistry.Results:At 72 h,the behavioral scores of Liangxue Tongyu group was significantly lower than those of the model group(P<0.05),and the scores continued to decrease with time.At 24 h,72 h and 120 h,the levels of TNF-αand IL-1 and the expression of NF-κB were lower in Liangxue Tongyu group than those in the model group(P<0.01).Conclusion:Liangxue Tongyu Decoction can regulate the activation of NF-κB pathway and inhibit the secretion of inflammatory factors in rats with intracerebral hemorrhage,thus reducing the occurrence of inflammatory reaction,reducing the cascade amplification effect of inflammation,improving the behavioral scores of rats with intracerebral hemorrhage and playing an anti-inflammatory role.

关 键 词：凉血通瘀方 脑出血 炎性因子 抗炎作用 

分 类 号：R285.5[医药卫生—中药学]