检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:丁大连 张建辉[2] 杨琨 蒋海燕[1] 亓卫东 时海波 刘芳[6] 李鹏[7] Richard Salvi[1] Ding Dalian;Zhang Jianhui;Yang Kun;Jiang Haiyan;Qi Weidong;Shi Haibo;Liu Fang;Li Peng;Richard Salvi(Center for Hearing and Deafness,State University of New York at Buffalo)
机构地区:[1]Center for Hearing and Deafness,State University of New York at Buffalo [2]成都市第三人民医院耳鼻咽喉头颈外科 [3]上海市第六人民医院耳鼻咽喉头颈外科 [4]武汉大学人民医院耳鼻咽喉头颈外科 [5]复旦大学附属华山医院耳鼻咽喉头颈外科 [6]北京医院耳鼻咽喉科国家老年医学中心 [7]中山大学附属第三医院耳鼻咽喉头颈外科
出 处:《听力学及言语疾病杂志》2020年第6期662-668,共7页Journal of Audiology and Speech Pathology
摘 要:目的探讨耳蜗发育过程中传出神经的发育分布。方法应用Tublin免疫组织化学方法标记出生后3~15天以及出生后4个月大鼠耳蜗基底膜上所有传入和传出神经纤维及其末梢的分布;应用酶组织化学或免疫组织化学方法观察出生后不同时间耳蜗基底膜上降钙素基因相关肽(calcitonin gene-related peptide,CGRP)和乙酰胆碱脂酶(AchE)的表达和分布。结果耳蜗传入神经纤维及末梢在大鼠出生后当天已经与内、外毛细胞建立起突触联系;从出生后第五天开始CGRP出现在内毛细胞周围及底部,但AchE标记的传出神经尚未进入Corti器,出生后第七天AchE才开始与CGRP同时出现在内侧螺旋束和隧道螺旋束的耳蜗传出神经纤维及末梢。结论 CGRP和乙酰胆碱出现在Corti器的时间均明显早于耳蜗传出神经末梢在耳蜗靶细胞的就位;在大鼠耳蜗发育早期出现在内毛细胞区域的CGRP和乙酰胆碱或许是由内毛细胞及其周围支持细胞分泌的一种诱导耳蜗传出神经纤维朝着耳蜗螺旋器趋向性生长的引导信号。Objective To study the developmental distribution of efferent nerves during cochlear development by evaluating the spatiotemporal distribution of efferent nerve markers, acetylcholinesterase(AchE) and calcitonin gene related peptide(CGRP) in rat cochlea from postnatal day to 15 days and 4 months after birth.Methods The innervation and distribution of afferent and efferent nervous system in the cochlear basilar membrane at different times after birth were assessed by Tublin immunochemistry. The enzyme histochemical and immunocytochemical techniques were also used to observe the expression and distribution of CGRP and AchE in cochlear efferent nerve fibers and synapse at different times after birth.Results The cochlear afferent nerve fibers and terminals had established synaptic connections with the inner and outer hair cells on the day after birth.CGRP began to appear in the region surrounding the inner hair cells on the fifth day after birth. However, AchE-labeled efferent nerve fibers had not entered the organ of Corti yet on the fifth day after birth. Unlike the CGRP, AchE appears expression was obserred simultaneously in the cochlear efferent fibers and terminals in both internal spiral bundle and tunnel spiral bundle from the seventh day after birth. It could be seen that CGRP and AchE appeared in the organ of Corti significantly later than the cochlear afferent innervation in the cochlear target cells in place. Conclusion The early expression of CGRP and acetylcholine in the region of inner hair cells may be guiding signals secreted by inner hair cells and surrounding support cells to induce the directional growth of efferent nerve fibers to the organ of Corti.
关 键 词:耳蜗发育 耳蜗传出神经 降钙素基因相关肽 乙酰胆碱酯酶
分 类 号:R339.16[医药卫生—人体生理学]
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:3.145.151.116