职业性铅接触工人相对端粒长度和端粒保护蛋白1结合蛋白1基因表达改变  

Changes of relative length of telomere and mRNA expression of telomere-binding protein POT1-interacting protein 1 in workers with occupational lead exposure

作　　者：张云霞 王团伟 孟钰 张光辉[2] 雷春萍 崔守明[4] 夏昭林 ZHANG Yunxia;WANG Tuanwei;MENG Yu;ZHANG Guanghui;LEI Chunping;CUI Shouming;XIA Zhaolin(Department of Occupational Health and Toxicology,School of Public Health,Fudan University;不详)

机构地区：[1]复旦大学公共卫生学院职业卫生与毒理学教研室,上海200032 [2]新乡医学院公共卫生学院,河南新乡453003 [3]重庆三峡医药高等专科学校公共卫生与管理学院,重庆404120 [4]河南省新乡市职业病防治研究所,河南新乡4530003

出　　处：《中国职业医学》2020年第4期414-417,423,共5页China Occupational Medicine

基　　金：国家自然科学基金面上项目(81872594)。

摘　　要：目的探讨职业性铅接触对工人周围血相对端粒长度(RTL)和端粒保护蛋白1结合蛋白1基因(TPP1)mRNA相对表达量的影响。方法采用单纯随机抽样方法,选择某铅蓄电池厂299名铅作业工人为接触组,以无职业性铅接触的68名行政后勤人员为对照组。采集2组人群周围血,采用石墨炉原子吸收光谱法检测血铅水平,采用实时荧光定量聚合酶链反应法检测RTL和TPP1 mRNA相对表达量。结果与对照组比较,接触组人群血铅水平升高[中位数(M):68.2 vs 266.1μg/L,P<0.01],RTL缩短(M:0.95 vs 0.69,P<0.01),TPP1 mRNA相对表达量降低(M:0.88 vs 0.51,P<0.01)。血铅水平与RTL、TPP1 mRNA相对表达量均呈负相关(Spearman相关系数分别为-0.17、-0.19,P<0.01);RTL与TPP1mRNA相对表达量呈正相关(Spearman相关系数=0.15,P<0.01)。多因素广义线性回归分析结果显示:铅接触者RTL缩短和TPP1 mRNA相对表达量下降的风险升高(P<0.01)。结论铅接触可导致工人周围血RTL缩短,TPP1 mRNA相对表达量降低;RTL缩短的机制可能是铅通过抑制TPP1 mRNA表达干扰端粒修复过程。Objective To explore the effect of occupational lead exposure on the relative telomere length(RTL)and mRNA expression of telomere-binding protein POT1-interacting protein 1(TPP1)in peripheral blood of workers.Methods A total of 303 workers exposed to lead were selected as the exposure group and 72 administrative logisticians personnel in the same factory as the control group using the simple random sampling method.Their peripheral blood samples were collected and were used to detect the blood lead level by Graphite furnace atomic absorption spectrometry.RTL and the relative expression of TPP1 mRNA by real-time quantitative polymerase chain reaction.Results The blood lead level of the exposure group was higher[Media(M):68.2 vs 266.1μg/L,P<0.01],the RTL was shorter(M:0.96 vs 0.70,P<0.01),and the relative mRNA expression of TPP1 was lower(M:0.92 vs 0.51,P<0.01)compared with the control group.Spearman correlation analysis results showed that the blood lead level were both negatively correlated with RTL[Spearman correlation coefficient(r_S)=-0.18,P<0.01],and the relative mRNA expression of TPP1(r_S=-0.19,P<0.01),while the RTL was positively correlated with the risk of RTL shortening and the relative mRNA expression of TPP1 decline was increased in lead exposure(P<0.01).Conclusion Lead exposure can shorten the RTL and reduce the relative mRNA expression of TPP1 in workers.The mechanism may be that lead interferes with telomere repair process by inhibiting the mRNA expression of TPP1.

关 键 词：铅 职业接触 工人 相对端粒长度 端粒结合蛋白 相关性 

分 类 号：R135[医药卫生—劳动卫生]