参草通脉颗粒对慢性心力衰竭大鼠抗心室重构机制的影响  

Effects of Shencao Tongmai Granules on the mechanism of anti-ventricular remodeling in rats with chronic heart failure

作　　者：刘晓蕾 张艳[2] 礼海[1] 王懿[1] 周雪[1] 陈菲 LIU Xiaolei;ZHANG Yan;LI Hai;WANG Yi;ZHOU Xue;CHEN Fei(Department of Medical,Shenyang Second Hospital of Traditional Chinese Medicine,Liaoning Province,Shenyang110015,China;Expert Consultation,Affiliated Hospital,Liaoning University of Traditional Chinese Medicine,Liaoning Province,Shenyang110015,China)

机构地区：[1]辽宁省沈阳市第二中医医院医务科,辽宁沈阳110015 [2]辽宁中医药大学附属医院专家门诊,辽宁沈阳110015

出　　处：《中国医药导报》2020年第30期8-11,共4页China Medical Herald

基　　金：国家自然科学基金资助项目(81774157)。

摘　　要：目的分析参草通脉颗粒对慢性心力衰竭(CHF)大鼠抗心室重构的作用机制。方法选取SPF级大鼠60只,随机抽取10只大鼠作为正常组,其余50只大鼠制备CHF大鼠模型,造模死亡9只,将剩余41只,随机分为模型组13只、参草通脉颗粒组14只、赖诺普利组14只。正常组和模型组大鼠不用药,赖诺普利组和参草通脉颗粒组大鼠连续给药42 d。采用逆转录聚合酶链反应(RT-PCR)和蛋白质印迹(WB)法测定基质金属蛋白酶-1(MMP-1)和金属蛋白酶组织抑制物-1(TIMP-1)的mRNA和蛋白表达。结果与模型组比较,参草通脉颗粒组和赖诺普利组MMP-1 mRNA和蛋白均降低,差异均有高度统计学意义(均P<0.01);与赖诺普利组比较,参草通脉颗粒组MMP-1 mRNA表达降低(P<0.05),而MMP-1蛋白表达升高(P<0.05)。与模型组比较,参草通脉颗粒组和赖诺普利组TIMP-1 mRNA和蛋白均增加,差异均有高度统计学意义(均P<0.01);与赖诺普利组比较,参草通脉颗粒组TIMP-1 mRNA和蛋白表达增加(P<0.05)。结论参草通脉颗粒治疗CHF大鼠效果显著,具有抗心室重构的作用。Objective To analyze the mechanism of Shencao Tongmai Granules on anti-ventricular remodeling in rats with chronic heart failure(CHF).Methods A total of 60 SPF rats were selected and ten rats were randomly taken as normal group.The other 50 rats were used to prepare the CHF rat model.In the process of modeling,nine rats failed to survive and the remaining 41 rats were divided into the model group(13 rats),the Shencao Tongmai Granules group(14 rats)and the Lisinopril group(14 rats)randomly.Rats in normal group and the model group were fed normally without drugs and rats in Lisinopril group and Shencao Tongmai Granules group were given drugs consecutively for 42 d.Reverse transcription PCR(RT-PCR)and Western blot(WB)were adopted to determine the mRNA and protein expression of matrix metalloproteinase-1(MMP-1)and tissue inhibitor of metalloproteinase-1(TIMP-1).Results Compared with the model group,the mRNA and protein expressions of MMP-1 in the Shencao Tongmai Granules group and Lisinopril group were reduced,and the differences were highly statistically significant(all P<0.01).Compared with the Lisinopril group,the MMP-1 mRNA expression of the Shencao Tongmai Granules group was decreased(P<0.05),while MMP-1 protein expression was increased(P<0.05).Compared with the model group,the mRNA and protein expressions of TIMP-1 in the Shencao Tongmai Granules group and Lisinopril group both increased,and the differences were highly statistically significant(all P<0.01).Compared with the Lisinopril group,the mRNA and protein expressions of TIMP-1 in the Shencao Tongmai Granules group were increased(P<0.05).Conclusion Shencao Tongmai Granules have a significant therapeutic effect on treating rats with CHF and can promote the mechanism of anti-ventricular remodeling.

关 键 词：慢性心力衰竭 参草通脉颗粒 基质金属蛋白酶-1 金属蛋白酶组织抑制物-1 

分 类 号：R285.5[医药卫生—中药学]