柚皮苷对胶质瘤细胞生长的抑制作用的机制研究  

Mechanisms of naringin inhibiting the growth of glioma cells

作　　者：王维[1] 赵子龙[1] 高伟[1] 张东勇[1] 张路阳[1] 包义君[1] WANG Wei;ZHAO Zilong;GAO Wei;ZHANG Dongyong;ZHANG Luyang;BAO Yijun(Department of Neurosurgery,the First Affiliated Hospital of China Medical,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院神经外科,辽宁沈阳110001

出　　处：《沈阳药科大学学报》2020年第9期815-818,共4页Journal of Shenyang Pharmaceutical University

基　　金：沈阳市科技计划项目(17-230-9-13)。

摘　　要：目的探讨柚皮苷对胶质瘤细胞生长的抑制作用。方法通过MTT实验检测不同浓度(0、0.1、0.5和1μmol·L^-1)柚皮苷对胶质瘤细胞(U87)生长的影响。采用流式细胞仪检测不同处理组对细胞周期的作用。采用Hochest 33258染色法检测柚皮苷对U87细胞凋亡的作用。通过Western blot试验检测柚皮苷对PI3K、PI3K p110、cyclin D1、bcl-2和bax蛋白的调节作用。结果对细胞处理48 h后,0.1μmol·L^-1和1μmol·L^-1柚皮苷处理组对U87细胞增殖抑制率分别是(20.35±0.07)%和(50.02±1.91)%。流式分析指出柚皮苷可以阻滞U87细胞的G1-S期进程。Hochest 33258染色证明柚皮苷可以促进U87细胞的凋亡。Western blot实验指出柚皮苷可以通过抑制PI3K p110、cyclin D1和bcl-2蛋白的表达,上调bax蛋白的表达来抑制U87细胞的生长。结论柚皮苷可以通过抑制PI3K的磷酸化抑制胶质瘤细胞的生长。Objective To investigate the inhibitory effect of naringin on the growth of glioma cells.Methods MTT assay was used to detect the effects of naringin on the growth of glioma cells(U87)at different concentrations(0μmol·L^-1,0.1μmol·L^-1,0.5μmol·L^-1 and 1μmol·L^-1).Flow cytometry was used to detect the effects of different treatment groups on cell cycle.Hochest 33258 staining was used to detect the apoptotic effect of naringin on U87 cells.The regulatory effects of naringin on PI3 K,PI3 K p110,cyclin D1,bcl-2 and bax proteins were detected by western blot.Results After 48 hours of treatment,the inhibition rates of 0.1μmol·L^-1 and 1μmol·L^-1 naringin on U87 cells were(20.35±0.07)%and(50.02±1.91)%,respectively.Flow pattern analysis indicated that naringin could block the G1-S phase of U87 cells.Hochest 33258 staining assay showed that naringin could promote U87 cell apoptosis.Western blot experiments indicated that naringin could inhibit the growth of U87 cells by inhibiting the expression of PI3 K p110,cyclin D1 and bcl-2 protein and up-regulating the expression of bax.Conclusion Naringin can inhibit the growth of glioma cells by inhibiting phosphorylation of PI3 K.

关 键 词：柚皮苷 胶质瘤 PI3K 生长 

分 类 号：R96[医药卫生—药理学]