AChE/PVP/Au@Pt/PVP/GC CME对甲基对硫磷含量的测定  

Determination of Methyl Parathion by AChE/PVP/Au@Pt/PVP/GC CME

作　　者：李正[1] 陈翊[1] 王玉 张玉[1] 张丽[1] LI Zheng;CHEN Yi;WANG Yu;ZHANG Yu;ZHANG Li(Hangzhou Yuhang Disease Control and Prevention Center,Hangzhou,Zhejiang,311100,China;Anhui Wuhu No.1 Middle School)

机构地区：[1]杭州市余杭区疾病预防控制中心,浙江杭州311100 [2]安徽省芜湖市第一中学

出　　处：《质量安全与检验检测》2020年第5期9-11,共3页QUALITY SAFETY INSPECTION AND TESTING

基　　金：杭州市科技发展计划项目(20150633B71);杭州市余杭区医疗卫生重点学科建设项目(2015004)。

摘　　要：为了快速测定磷酸盐缓冲溶液(PBS)中的甲基对硫磷,本文通过层层自组装法制备纳米粒子-酶传感器,用此法对甲基对硫磷进行测定。结果显示,该传感器对甲基对硫磷浓度的测定线性范围为1.5×10^-5~1.0×10^-3 mol/L,相关系数R≥0.998,测定PBS中甲基对硫磷的检出限达5.0×10^-6 mol/L。双金属纳米粒子修饰电极测定甲基对硫磷的线性范围较宽,检出限较低,且稳定性和选择性良好。In order to quickly determine the methyl parathion in the phosphate buffer solution(PBS),this paper uses the layer-by-layer self-assembly method to prepare a nanoparticle-enzyme sensor,and uses this method to determine the methyl parathion.The results show that the linear range of the sensor for the concentration of methyl parathion is 1.5×10^-5—1.0×10^-3 mol/L,and the correlation coefficient R≥0.998,the detection limit of methyl parathion in PBS is 5.0×10^-6 mol/L.The bimetallic nanoparticle modified electrode has a wider linear range for the determination of methyl parathion,a lower detection limit,and good stability and selectivity.

关 键 词：甲基对硫磷 金核铂壳纳米粒子 乙酰胆碱酯酶 酶传感器 

分 类 号：O657.1[理学—分析化学]