蔗糖浓度、温度及光照强度对试管薯诱导的影响  

Effects of Sucrose Concentration Temperature and Light Intensity on Induction Effect of Potato Microtuber

作　　者：邱甜 牛力立 朱江 蔡甫格 严云刚 QIU Tian;NIU Lili;ZHU Jiang;CAI Fuge;YAN Yungang(School of Agricultural,Anshun University,Anshun 561000,Guizhou,China;Anshun A cademy of Agricutural Sciences,Anshun 561000,Guizhou,China)

机构地区：[1]安顺学院农学院,贵州安顺561000 [2]安顺市农业科学院,贵州安顺561000

出　　处：《安顺学院学报》2020年第5期121-123,135,共4页Journal of Anshun University

基　　金：贵州省教育厅青年科技人才成长项目“脱毒马铃薯试管薯繁育与规模化生产体系研究”(黔教合KY[2016]274号);安顺市科技局项目“安顺市薯芋科技创新人才团队”(安市科人[2019]2号)。

摘　　要：在马铃薯试管薯诱导的过程中,蔗糖浓度、温度和光照强度对试管薯的诱导形成具有较大影响。本试验通过研究不同蔗糖浓度、温度和光照强度对马铃薯试管薯诱导效果的影响,旨在筛选出一步法诱导最适的蔗糖浓度、温度和光照强度,用于指导马铃薯试管薯的工厂化生产。结果表明:在试管薯诱导培养过程中,控制蔗糖浓度60-80g·L^-1、温度18-20℃、光照强度80-100μmol·m^-2·s^-1时,能获得一个较好的马铃薯试管薯结薯数和结薯重,以蔗糖浓度为60 g·L^-1,温度18℃,光照强度为80μmol·m^-2·s^-1和蔗糖浓度60 g·L^-1,温度20℃,光照强度为100μmol·m^-2·s^-1的诱导4周后,黑暗诱导4周的诱导效果最佳。In the process of potato tuber induction,sucrose concentration,temperature and light intensity have a greater influence on the formation of tuber tubers.By studying the effect of different sucrose concentration,temperature and light intensity on the induction of potato test tube potatoes,it took aim to screen out the optimal sucrose concentration,temperature and light intensity induced,which could guide the factory production of potato test tube potatoes.The results show that during the induction culture of test tube potatoes,the number and weight of potato tubers can be higher when the sucrose concentration is controlled at 60-80g·L^-1,the temperature is 18-20℃,and the light intensity is 80-100μmol·m^-2·s^-1,And the number and weight of Potato tubers could be highest with sucrose concentration of 60 g·L^-1,temperature 18℃,light intensity of 80mol·m^-2·s^-1 and sucrose concentration 60 g·L^-1,temperature 20℃.After 4 weeks of induction with a light intensity of 100 mol·m^-2·s^-1,the induction effect of dark induction for 4 weeks was the best.

关 键 词：马铃薯 试管薯 蔗糖 温度 光照强度 诱导 

分 类 号：S532[农业科学—作物学]