基于网络药理学分析甘味药治疗肾实证的作用机制  

An Analysis of the Mechanism of Sweet Medicinals in Treating Kidney Excess Syndrome Based on Network Pharmacology

作　　者：范珉珏 贾育新 柴瑞婷 FAN Min-jue;JIA Yu-xin;CHAI Rui-ting(Key Laboratory of Dunhuang Medicine,Ministry of Education of Gansu University of Chinese Medicine,Lanzhou,Gansu,China,730000)

机构地区：[1]甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室,甘肃兰州730000

出　　处：《河南中医》2020年第11期1756-1762,共7页Henan Traditional Chinese Medicine

摘　　要：目的:以临床治疗肾实证应用较多的甘味药物甘草、茯苓为代表,从其靶点、信号通路以及分子结构的角度,分析甘味药泻肾作用的可能机制。方法:从中国知网检索中医药治疗肾实证的文献,录入到中医传承辅助平台(TCMMISS,V2.5),进行药物频次和性味频率分析。将分析结果中的核心甘味药录入至在线中医药生物信息学分析工具BATMAN-TCM,对核心甘味药与潜在作用靶点之间的关系进行分析。结果:筛选出符合条件的方剂20首,包含中药72味,苦味药占35%,甘味药占34%,甘味药中甘草和茯苓出现的频率较高。甘草参与防治肾实证相关疾病的通路包括:醛固酮调节的钠离子重吸收、内分泌和其他因子调节的钙离子重吸收以及近端小管碳酸氢盐重吸收;茯苓参与防治肾实证相关疾病的通路包括:醛固酮调节的钠离子重吸收、内分泌和其他因子调节的钙离子重吸收。结论:甘味药可能通过AR、VDR、ADORA1、HTR2C以及ADORA2A等5个靶点,醛固酮与内分泌和其他因子对钠、钙离子重吸收的信号通路等2个途径,来调节水液代谢,从而发挥对肾实证的治疗作用。Objective:To analyze the possible mechanism of kidney-purging effect of sweet medicinals from the perspective of their target,signal pathway and molecular structure by taking sweet Gancao(Radix et Rhizoma Glycyrrhizae)and Fuling(Poria)which are widely used in clinical treatment of kidney excess syndrome.Methods:The literatures about kidney excess syndrome treated by TCM were retrieved from CNKI and entered into TCM inheritance platform(TCMMISS,V2.5)to analyze the frequency of medicinals and their natures and tastes.The core sweet medicinals in the analysis results were input into BATMAN-TCM,the online bioinformatics analysis tool of TCM,and the relationship between core sweet medicinals and potential targets was analyzed.Results:A total of 20 formulas were screened out,including 72 kinds of Chinese medicinals,35%bitter medicinals and 34%sweet medicinals.Gancao and Fuling appeared frequently in sweet medicinals.The pathways of Gancao in the prevention and treatment of kidney excess syndrome related diseases included:aldosterone regulated sodium reabsorption,endocrine and other factors regulated calcium reabsorption,and proximal tubular bicarbonate reabsorption.The pathways of Fuling involved in the prevention and treatment of kidney syndrome related diseases include aldosterone regulated sodium reabsorption,endocrine and other factors regulated calcium reabsorption.Conclusion:Sweet medicinals may regulate water metabolism through five targets,such as AR,VDR,ADORA1,HTR2 C and ADORA2 A,and two pathways of aldosterone,endocrine and other factors′reabsorption of sodium and calcium ions,so as to play a therapeutic role in kidney excess syndrome.

关 键 词：肾实证 甘味药 甘草 茯苓 网络药理学 

分 类 号：R282.7[医药卫生—中药学]