电针“水沟”对脑梗死大鼠脑动脉血管平滑肌中可溶性鸟苷酸环化酶及蛋白激酶G表达的影响  

Effects of electroacupuncture“Shuigou”on expression of soluble guanylate cyclase(sGC)and protein kinase G(PKG)in vascular smooth muscle of cerebral artery in rats with cerebral infarction

作　　者：徐彦龙[1] 徐秀梅[2] 杨正飞 郭茂娟 姜希娟 XU Yan-long;XU Xiu-mei;YANG Zheng-fei;GUO Mao-juan;JIANG Xi-juan(Department of Acupuncture and Moxibustion,Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,China;Department of Rehabilitation Medicine,Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,China;Graduate School,Tianjin University of Traditional Chinese Medicine,Tianjin 300193;College of Integrated Chinese and Western Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 300193)

机构地区：[1]甘肃省中医院针灸一科,兰州730050 [2]甘肃省中医院康复医学科,兰州730050 [3]天津中医药大学研究生院,天津300193 [4]天津中医药大学中西医结合学院,天津300193

出　　处：《针刺研究》2020年第10期789-792,805,共5页Acupuncture Research

基　　金：国家自然科学基金项目(No.81560794)。

摘　　要：目的:观察电针“水沟”对脑梗死大鼠脑动脉血管平滑肌可溶性鸟苷酸环化酶(sGC)、蛋白激酶G(PKG)及环磷酸鸟苷(cGMP)变化的动态调节规律。方法:Wistar大鼠随机分为空白组(10只)、假手术组(40只)、模型组(40只)、电针组(40只),后3组在造模后又分为3、6、12、24h4个时相组,每组10只。以线栓法复制脑梗死大鼠模型。电针组在造模后即刻针刺“水沟”,电针刺激20min。各组动物均按时相处死,应用蛋白免疫印迹法、酶联免疫吸附法分别检测脑动脉血管平滑肌sGC、PKG及cGMP的变化。结果:大鼠脑梗死后24h内,与空白组和假手术组比较,模型组3h时相的sGC表达水平显著下调(P<0.01),且sGC的整体表达量随梗死时间的延长有逐渐升高的趋势,3、6h时相的PKG表达量显著下调(P<0.05,P<0.01),各时相的cGMP含量均显著下降(P<0.01)。与同时相模型组比较,电针组3h时相的sGC表达水平明显上调(P<0.05),3、6h时相的PKG表达水平上调(P<0.05),3、6h的cGMP含量增加(P<0.05)。结论:针刺干预能显著抑制脑梗死模型大鼠脑动脉血管平滑肌sGC、PKG、cGMP表达下调,这对于抑制缺血后脑动脉血管平滑肌痉挛,保持血管功能和状态的正常,从而增加脑梗死区周围血流灌注具有重要作用,但针刺效应具有一定时效性。Objective To observe the effect of electroacupuncture(EA)on the expression of soluble guanylate cyclase(sGC),cyclic guanosine phosphate(cGMP)and protein kinase G(PKG)of cerebral vascular smooth muscle in cerebral infarction(CI)rats,so as to study its dynamic regulation mechanism.Methods Male Wistar rats were randomly divided into normal control(n=10),sham operation(n=40),model(n=40),and EA(n=40)groups,and the latter three groups were further divided into 3,6,12and 24hsubgroups(n=10in each subgroup).The CI model was established by occlusion of the middle cerebral artery(MCAO).EA(15Hz,2mA)was applied to“Shuigou”(GV26)for 20min.The cGMP,sGC and PKG activity and expression levels in the vascular smooth muscle of cerebral artery were detected using ELISA and Western blot,respectively.Results After modeling,the immunoactivity and activities of sGC at 3h,PKG at 3and 6hand cGMP from 3hto 24hwere obviously decreased in the model group relevant to the normal control and sham-operation groups(P<0.05,P<0.01).After the intervention,the expression levels and activities of sGC at 3h,PKG at 3and 6hand cGMP at 3and 6hwere apparently upregulated in the EA group relevant to the model group(P<0.05).Conclusion EA intervention can significantly inhibit the down-regulation of sGC,PKG and cGMP expression of cerebral artery smooth muscle in MCAO model rats,which plays an important role in inhibiting cerebral artery smooth muscle spasm after ischemia,maintaining normal vascular function and state,and thus increasing blood perfusion around cerebral infarction area.However,acupuncture effect has a certain time-effectiveness.

关 键 词：脑梗死 电针 可溶性鸟苷酸环化酶 环磷酸鸟苷 蛋白激酶G 血管平滑肌 

分 类 号：R245.97[医药卫生—针灸推拿学]