益肾骨康方对肺腺癌A549细胞增殖、迁移、黏附、侵袭的影响  

Effect of Yishen Gukang Prescription on Proliferation,Migration,Adhesion and Invasion of Lung Cancer A549 Cells

作　　者：何理玉 梁敏 芦殿荣[1] 冯利 HE Liyu;LIANG Min;LU Dianrong;FENG Li(Wangjing Hospital,China Academy of Chinese Medical Sciences,Beijing China 100102;National Cancer Center/National Cancer Research Center/Tumor Hospital of Peking Union Medical College,China Academy of Chinese Medical Sciences,Beijing China 100021)

机构地区：[1]中国中医科学院望京医院,北京100102 [2]国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院,北京100021

出　　处：《中医学报》2020年第11期2392-2396,共5页Acta Chinese Medicine

基　　金：国家自然科学基金项目(81603598)。

摘　　要：目的:观察益肾骨康方对肺腺癌A549细胞增殖、迁移、黏附、侵袭的影响。方法:以0.1 g·L-1、0.5 g·L-1、1 g·L-1、2 g·L-1、4 g·L-1、6 g·L-1、8 g·L-1的益肾骨康方作用于人肺腺癌A549细胞株,分别于24 h、48 h、72 h检测不同质量浓度的益肾骨康方对肺癌A549细胞增殖能力的影响,依据细胞存活率计算半数抑制浓度(IC50)。划痕实验、黏附实验、Transwell侵袭实验,分为对照组,益肾骨康方低剂量组、高剂量组,环磷酰胺组,分别干预肺癌A549细胞24 h和(或)48 h后检测肺癌A549细胞的迁移、黏附、侵袭能力。结果:益肾骨康方可以抑制肺癌A549细胞的增殖,质量浓度为1 g·L-1、2 g·L-1、4 g·L-1时,对A549细胞的抑制程度分别随时间和质量浓度增加而增加。将1 g·L-1作为益肾骨康方低剂量组干预浓度;将24 h半数抑制浓度4.18 g·L-1(IC50)作为益肾骨康方高剂量组的干预浓度。益肾骨康组(低剂量组、高剂量组)、环磷酰胺组细胞迁移率、细胞侵袭数低于对照组(P<0.01);益肾骨康方高剂量组、环磷酰胺组细胞黏附率低于对照组(P<0.01)。益肾骨康方高剂量组细胞迁移率、黏附率低于益肾骨康方低剂量组(P<0.05或P<0.01)。益肾骨康组细胞迁移率高于环磷酰胺组(P<0.01),益肾骨康方高剂量组细胞黏附率低于环磷酰胺组(P<0.01)。结论:益肾骨康方可以抑制肺癌A549细胞的增殖、迁移、黏附、侵袭能力。Objective:To investigate the intervention effect of Yishen Gukang prescription on tumor metastasis.Methods:Human lung adenocarcinoma cell line A549 was treated with Yishen Gukang prescription at gradient concentrations of 0.1 g·L-1,0.5 g·L-1,1 g·L-1,2 g·L-1,4 g·L-1,6 g·L-1 and 8 g·L-1,respectively.The effects of Yishen Gukang prescription on proliferation of lung cancer A549 cells were detected at 24 h,48 h and 72 h,respectively.The half inhibitory concentration(IC50)was calculated according to the cell survival rate.Scratch test,adhesion test and Transwell invasion test were used to divide them into control group,Yishen Gukang prescription low-dose group,high-dose group and cyclophosphamide group.The migration,adhesion and invasion ability of lung cancer A549 cells were detected after 24 h and/or 48 h intervention.Results:Yishen Gukang prescription can inhibit the proliferation of lung cancer A549 cells.When the mass concentration is 1 g·L-1,2 g·L-1 and 4 g·L-1,the inhibition degree of A549 cells increases with the increase of time and mass concentration.The intervention concentration of 1 g·L-1 was used as the intervention concentration of the low dose group of Yishen Gukang prescription,and the half inhibitory concentration of 24 h was 4.18 g·L-1(IC50)as the intervention concentration of the high dose group.The cell migration rate and cell invasion number of Yishen Gukang prescription group(low-dose group,high-dose group)and cyclophosphamide group were lower than those of the control group(P<0.01);the cell adhesion rate of high-dose Yishen Gukang prescription group and cyclophosphamide group was lower than that of the control group(P<0.01).The cell migration rate and adhesion rate in the high-dose group were lower than those in the low-dose group(P<0.05 or P<0.01).The cell migration rate of Yishen Gukang prescription group was higher than that of cyclophosphamide group(P<0.01),and the cell adhesion rate of high-dose Yishen Gukang prescription group was lower than that of cyclophosphamide group(P<0.

关 键 词：肺癌 益肾骨康方 A549细胞 肿瘤转移 细胞增殖 细胞迁移 细胞黏附 细胞侵袭 

分 类 号：R285.5[医药卫生—中药学]