Dracocephalum palmatum Stephan extract induces apoptosis in human prostate cancer cells via the caspase-8-mediated extrinsic pathway  

作　　者：LEE Se-Eun OKHLOPKOVA Zhanna LIM Chiyeon CHO Suin 

机构地区：[1]School of Korean Medicine,Pusan National University,Yangsan-si 50612,Republic of Korea [2]Department of Biology,North-Eastern Federal University,Yakutsk 677027,Russia [3]College of Medicine,Dongguk University,Gyeonggi-do 10326,Republic of Korea

出　　处：《Chinese Journal of Natural Medicines》2020年第10期793-800,共8页中国天然药物（英文版）

摘　　要：Dracocephalum palmatum Stephan is a medicinal plant traditionally used by nomadic people in Eastern Russia;however,research on this plant is currently limited.Recently,although studies have been conducted on the constituents of this plant and their antioxidant effects,data on its various pharmacological activities are still lacking.Thus,this study examined the anticancer potential of the dried leaves of D.palmatum S.(DpL)using human prostate cancer PC-3 cells.The antioxidant potential of DpL was evaluated by estimating the total flavonoid and total phenolic content(TFC and TPC,respectively).Additionally,we investigated the effects of the DpL ethyl acetate fraction(DpLE)on cell proliferation,intracellular reactive oxygen species(ROS)generation,apoptosis,and cell cycle arrest in this cell line.The expression levels of superoxide dismutase(SOD)-1,SOD-2,B-cell lymphoma 2(Bcl-2)and Bcl-2 associated X(Bax)ratio,phospho-protein kinase B(p-AKT),cleaved caspase-8,poly adenosine diphosphate(ADP)ribose polymerase(PARP),and cleaved-PARP were evaluated by western blotting.The results indicated that DpLE causes apoptosis and exerts intracellular ROS-independent anticancer effects on prostate cancer cells,associated with increased SOD-2,cleaved caspase-8,and cleaved-PARP expression and inhibited p-AKT signaling.Thus,DpLE may be a potential resource for the development of promising chemotherapeutic agents for prostate cancer.

关 键 词：Dracocephalum palmatum Stephan Prostate cancer APOPTOSIS CASPASE-8 

分 类 号：R965[医药卫生—药理学]