复合诱变野生酵母ZZ-46选育高产油脂菌株  

Improving microbial oil yield in Trichosporon dermatis by mutagenesis

作　　者：周秋利 顾喆 龙凌凤 郭书贤 刘汝宽[3] 孙海彦[4] 孙付保 ZHOU Qiuli;GU Zhe;LONG Lingfeng;GUO Shuxian;LIU Rukuan;SUN Haiyan;SUN Fubao(Key Laboratory of Carbohydrate Chemistry and Biotechnology of MOE,School of Biotechnology,Jiangnan University,Wuxi 214122,China;Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology,School of Biotechnology,Nanyang University of Technology,Nanyang 473004,China;State Key Laboratory of Utilization of Woody Oil Resource,Hunan Academy of Forestry,Changsha 410004,China;Hainan Key Laboratory of Tropical Microbe Resources,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]江南大学生物工程学院糖化学与生物技术教育部重点实验室,江苏无锡214122 [2]南阳理工学院生物与化学工程学院河南省工业微生物资源与发酵技术重点实验室,河南南阳473004 [3]湖南省林业科学院省部共建木本油料资源利用国家重点实验室,湖南长沙410004 [4]中国热带农业科学院热带生物技术研究所海南省热带微生物资源重点实验室,海南海口571101

出　　处：《食品与发酵工业》2020年第21期16-22,共7页Food and Fermentation Industries

基　　金：国家自然科学基金资助项目(21776114);国家木薯产业技术体系岗位科学家项目(CARS-11-HNSHY);江苏省“六大人才高峰”高层次人才项目(XNY-010);江苏省自然科学基金资助项目(BK20181347)。

摘　　要：为获得能利用葡萄糖/木糖混合糖液的高产油脂菌种,以野生酵母菌株Trichosporon dermatis ZZ-46为出发菌进行常压室温等离子体(atmospheric room temperature plasma,ARTP)-硫酸二乙酯(diethyl sulfate,DES)复合诱变,并分别以浅蓝菌素和氯化三苯基四氮唑(triphenyl tetrazolium chloride,TTC)分别为筛选因子结合尼罗红荧光染色进行高通量筛选,最终选育出1株高产菌株L7。该菌以混合糖为碳源进行摇瓶复筛后,生物量、油脂产量和油脂含量分别达到22.7 g/L、11.4 g/L和50.5%,较出发菌株分别提高12.6%、33.2%和7.8%;且该菌具有良好的遗传稳定性,其脂肪酸组成与出发菌相同,与植物油相似。结果表明,该菌具有较好利用混合糖液生产生物柴油的潜力。To obtain a high-yield oil strain utilizing glucose and xylose,the wild yeast Trichosporon dermatis ZZ-46 was mutated by using atmospheric and room temperature plasma mutagenesis and diethyl sulfate treatment.A high-yield strain T.dermatis L7 was finally obtained after a high-throughput screening.With the mixed sugars used as a carbon source,the cell biomass,oil production,and oil content of this mutated strain reached 22.7 g/L,11.4 g/L,and 50.5%,respectively,which were 12.6%,33.2%,and 7.8%higher than that of parent strain,correspondingly.The mutant presented well genetic stability,and the fatty acid composition was the same as that of parent strain,which was extremely similar to vegetable oil.The mutant strain obtained in this study has potential in biodiesel production from lignocellulose hydrolysate predominately containing glucose and xylose.

关 键 词：油脂酵母Trichosporon dermatis L7 常压室温等离子体 硫酸二乙酯 微生物油脂 诱变选育 混合糖 

分 类 号：TE667[石油与天然气工程—油气加工工程]